Genotyping of Strawberry (Fragaria x ananassa Duch.) Cultivars by DNA Markers: Interlaboratory Study

被引：0
|
作者：
Kunihisa, Miyuki [1 ]
Ueda, Hiroshi [2 ]
Fukino, Nobuko [2 ]
Matsumoto, Satoru [2 ]
机构：
[1] Natl Inst Vegetable & Tea Sci, Tsukuba, Ibaraki 3058666, Japan
[2] Natl Inst Vegetable & Tea Sci, Tsu, Mie 5142392, Japan
来源：
JOURNAL OF AOAC INTERNATIONAL | 2009年 / 92卷 / 03期
关键词：
CAPS MARKERS; IDENTIFICATION;
D O I：
10.1093/jaoac/92.3.896
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Fourteen Japanese laboratories validated the reproducibility of genotyping by 25 cleavage amplified polymorphic sequence (CAPS) markers for discrimination of strawberry (Fragaria x ananassa Duch.) cultivars. Both the sensitivity and specificity rate of 12 markers were 100%, those of another 12 were >95%, and those of I were >90%. These results indicate that the method of genotyping by the CAPS markers was highly reproducible and could provide a useful basis for practical identification of strawberry cultivars. This is the first report of the statistical validation of crop genotyping by DNA markers.
引用
收藏
页码：896 / 906
页数：11
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