Real-time measurement of complex refractive indices with surface plasmon resonance

被引:10
|
作者
Filion-Cote, Sandrine [1 ]
Tabrizian, Maryam [2 ,3 ]
Kirk, Andrew G. [1 ]
机构
[1] McGill Univ, Dept Elect & Comp Engn, Photon Syst Grp, McConnell Engn Bldg, Montreal, PQ H3A 0E9, Canada
[2] McGill Univ, Dept Biomed Engn, Duff Med Bldg, Montreal, PQ H3A 2B4, Canada
[3] McGill Univ, Fac Dent, Strathcona Anat & Dent Bldg, Montreal, PQ H3A 0C7, Canada
来源
基金
加拿大自然科学与工程研究理事会;
关键词
Surface plasmon resonance; Biosensing; Data analysis; Imaginary part; Liposomes; SENSORS;
D O I
10.1016/j.snb.2017.02.004
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Surface plasmon resonance (SPR) is an attractive method for the measurement of refractive indices due to its high accuracy and its broad range of applications. However, the use of this technique for the measurement of complex refractive indices in real-time has not yet been demonstrated. This article discusses and demonstrates how an SPR biosensor can be used to accurately measure both the real and imaginary components of the refractive index of a sample in real-time. The theory of the data analysis method, the calibration of the instrument as well as the application of the new technique to measure the complex refractive index of liposomes are presented. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码:747 / 752
页数:6
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