Translation of the mRNA for the sporulation gene spoIIID of Bacillus subtilis is dependent upon translation of a small upstream open reading frame

被引：6
|
作者：
Decatur, A [1 ]
McMurry, MT [1 ]
Kunkel, BN [1 ]
Losick, R [1 ]
机构：
[1] HARVARD UNIV, BIOL LABS, DEPT MOL & CELLULAR BIOL, CAMBRIDGE, MA 02138 USA
来源：
JOURNAL OF BACTERIOLOGY | 1997年 / 179卷 / 04期
关键词：
D O I：
10.1128/jb.179.4.1324-1328.1997
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We report the existence of a small open reading frame (usd) that is located between the promoter and coding sequence for the sporulation gene spoIIID in Bacillus subtilis. The mRNA from the usd-spoIIID operon contains an inverted repeat sequence that is predicted to form a stem-loop structure that would sequester the ribosome binding site for spoIIID. A mutation eliminating the ribosome binding site for the upstream open reading frame caused an oligosporogenous phenotype and interfered with the translation, but not the transcription, of the downstream gene spoIIID. We propose that efficient synthesis of SpoIIID requires that the putative stem-loop structure be disrupted bg translation through the upstream open reading frame.
引用
收藏
页码：1324 / 1328
页数：5
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