The relationship between seropositivity and tissue cysts in sheep naturally infected with Toxoplasma gondii

被引：12
|
作者：
Yildiz, Kader [1 ]
Kul, Oguz [2 ]
Gokpinar, Sami [1 ]
Atmaca, Hasan Tarik [2 ]
Gencay, Yilmaz Emre [1 ,3 ]
Gazyagci, Aycan Nuriye [1 ]
Babur, Cahit [4 ]
Gurcan, Ismayil Safa [5 ]
机构：
[1] Kirikkale Univ, Fac Vet Med, Dept Parasitol, Kirikkale, Turkey
[2] Kirikkale Univ, Fac Vet Med, Dept Pathol, Kirikkale, Turkey
[3] Kirikkale Univ, Fac Vet Med, Dept Food Hyg & Technol, Kirikkale, Turkey
[4] Publ Hlth Inst Turkey, Ankara, Turkey
[5] Ankara Univ, Fac Vet Med, Dept Biostat, TR-06100 Ankara, Turkey
来源：
TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES | 2014年 / 38卷 / 02期
关键词：
Toxoplasma gondii; tissue cyst; percoll gradient centrifugation; tissue microarray; serology; sheep; Turkey; HUMAN CONSUMPTION; HIGH PREVALENCE; MEAT; SEROPREVALENCE; GENOTYPES; STORE;
D O I：
10.3906/vet-1308-49
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Skeletal muscles (tongue, masseter, leg, intercostal, and diaphragmatic muscles) and brain samples of 100 sheep at slaughter were analysed for the presence of T. gondii tissue cysts along with serum IgG titres. Two methods of isolation by percoll gradient centrifugation and tissue microarray (TMA) technique with immunoperoxidase staining were used. Seropositivity was detected in 88% (88/100) of sheep sera analysed by indirect fluorescent antibody test. Tissue cysts were observed in 46 (52.3%, 46/88) and 15 (17%, 15/88) of the seropositive sheep with the isolation technique and TMA and immunoperoxidase staining, respectively. The diameters of the tissue cysts were 25-58 x 25-62 (mean 34 x 36) mu m. The relationship between the presence of tissue cysts and seropositivity in sheep was statistically significant at 1/16 (P < 0.01) and at 1/64 and 1/128 (P < 0.001) serum dilutions.
引用
收藏
页码：169 / 175
页数：7
相关论文
共 50 条
  • [1] Molecular evidence of Toxoplasma gondii from the tissue and blood of naturally infected sheep
    Abdullah, Shadan Hassan
    [J]. ITALIAN JOURNAL OF FOOD SAFETY, 2024, 13 (02):
  • [2] Toxoplasma gondii detection in the semen of naturally infected sheep
    Moraes, Erica P. B. X.
    Faria, Eduardo B.
    Batista, Andre M.
    Freitas, Antonio Carlos
    Silva, Jean Carlos R.
    Albuquerque, Pedro Paulo F.
    Mota, Rinaldo A.
    [J]. PESQUISA VETERINARIA BRASILEIRA, 2010, 30 (11): : 915 - 917
  • [3] Distribution of Toxoplasma gondii Tissue Cysts in Shoulder Muscles of Naturally Infected Goats and Lambs
    Rani, Surabhi
    Cerqueira-Cezar, Camila K.
    Murata, Fernando H. A.
    Kwok, Oliver C. H.
    Dubey, Jitender P.
    Pradhan, Abani K.
    [J]. JOURNAL OF FOOD PROTECTION, 2020, 83 (08) : 1396 - 1401
  • [4] TOXOPLASMA-GONDII CYSTS IN PLACENTAS OF EXPERIMENTALLY INFECTED SHEEP
    DUBEY, JP
    [J]. AMERICAN JOURNAL OF VETERINARY RESEARCH, 1987, 48 (03) : 352 - 353
  • [5] Immunodetection of Toxoplasma gondii in placenta tissue of naturally infected goats
    Mesquita, E. P.
    Oliveira, J. M. B.
    Silva, G. M.
    Torres, S. M.
    Oliveira, A. A. F.
    Silva Junior, V. A.
    Mota, R. A.
    Amorim, M. J. A. A. L.
    [J]. ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA, 2019, 71 (01) : 86 - 92
  • [6] Detection of Toxoplasma gondii DNA in naturally infected sheep's milk
    de Santana Rocha, D.
    de Sousa Moura, R. L.
    Maciel, B. M.
    Guimaraes, L. A.
    O'dwyer, H. N. S.
    Munhoz, A. D.
    Albuquerque, G. R.
    [J]. GENETICS AND MOLECULAR RESEARCH, 2015, 14 (03) : 8658 - 8662
  • [7] Relationship between Toxoplasma gondii seropositivity and depression in children and adolescents
    Sapmaz, Sermin Yalin
    Sen, Semra
    Ozkan, Yekta
    Kandemir, Hasan
    [J]. PSYCHIATRY RESEARCH, 2019, 278 : 263 - 267
  • [8] Toxoplasma Gondii: A Review of the Relationship Between Seropositivity and Psychiatric Morbidity
    Adams, David P.
    Guerin, Carol
    [J]. BIOLOGICAL PSYCHIATRY, 2014, 75 (09) : 68S - 68S
  • [9] Determination of seropositivity for Toxoplasma gondii in sheep in Sanliurfa province
    Sevgili, M
    Babür, C
    Nalbantoglu, S
    Karas, G
    Vatansever, Z
    [J]. TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, 2005, 29 (01): : 107 - 111
  • [10] Methods for Inactivation of Toxoplasma gondii Cysts in Meat and Tissues of Experimentally Infected Sheep
    El-Nawawi, Fathi A.
    Tawfik, Mohamed A.
    Shaapan, Raafat M.
    [J]. FOODBORNE PATHOGENS AND DISEASE, 2008, 5 (05) : 687 - 690
12345