Recruitment of Condensin to Replication Origin Regions by ParB/SpoOJ Promotes Chromosome Segregation in B. subtilis

被引：242

作者：

Gruber, Stephan ^{[1
]}

Errington, Jeff ^{[1
]}

机构：

[1] Univ Newcastle, Inst Cell & Mol Biosci, Ctr Bacterial Cell Biol, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England

来源：

CELL | 2009年 / 137卷 / 04期

基金：

英国生物技术与生命科学研究理事会;

关键词：

BACILLUS-SUBTILIS; SMC PROTEIN; ESCHERICHIA-COLI; DNA-BINDING; CAULOBACTER-CRESCENTUS; STRUCTURAL MAINTENANCE; SPO0J; BACTERIAL; PARB; INITIATION;

D O I：

10.1016/j.cell.2009.02.035

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proper segregation of DNA replication products is essential in all cells. In Bacillus subtilis, two protein complexes have been implicated in this process: the ParAB homologs, Soj and SpoOJ, and the bacterial Smc/ScpAB complex, also called condensin. Here we demonstrate that Smc is highly enriched in the region around the origin of replication, specifically near parS sites to which SpoOJ binds and at highly transcribed genes. Furthermore, we find that efficient recruitment of Smc to a large region around the origin of replication depends on the presence of SpoOJ. We show that SpoOJ performs two independent functions: regulation of initiation of DNA replication via Soj and promotion of chromosome segregation by Smc recruitment. Our results demonstrate a direct functional interaction between two widely conserved systems involved in chromosome replication and segregation.

引用

页码：685 / 696

页数：12

共 12 条

[1] Recruitment of SMC by ParB-parS Organizes the Origin Region and Promotes Efficient Chromosome Segregation
Sullivan, Nora L.
Marquis, Kathleen A.
Rudner, David Z.
CELL, 2009, 137 (04) : 697 - 707
[2] Chromosome segregation in B. subtilis is highly heterogeneous
Nina El Najjar
Peter L. Graumann
BMC Research Notes, 13
[3] Chromosome remodelling and DNA replication in B. subtilis
Soultanas, P.
FEBS JOURNAL, 2006, 273 : 53 - 53
[4] Interlinked Sister Chromosomes Arise in the Absence of Condensin during Fast Replication in B. subtilis
Gruber, Stephan
Veening, Jan-Willem
Bach, Juri
Blettinger, Martin
Bramkamp, Marc
Errington, Jeff
CURRENT BIOLOGY, 2014, 24 (03) : 293 - 298
[5] Direct evidence for active segregation of oriC regions of the Bacillus subtilis chromosome and co-localization with the SpoOJ partitioning protein
Lewis, PJ
Errington, J
MOLECULAR MICROBIOLOGY, 1997, 25 (05) : 945 - 954
[6] Chromosome remodelling by SMC/Condensin in B. subtilis is regulated by monomeric Soj/ParA during growth and sporulation
Roberts, David M.
Anchimiuk, Anna
Kloosterman, Tomas G.
Murray, Heath
Wu, Ling Juan
Gruber, Stephan
Errington, Jeff
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2022, 119 (41)
[7] Bacillus subtilis SMC is required for proper arrangement of the chromosome and for efficient segregation of replication termini but not for bipolar movement of newly duplicated origin regions
Graumann, PL
JOURNAL OF BACTERIOLOGY, 2000, 182 (22) : 6463 - 6471
[8] STRUCTURE OF REPLICATION ORIGIN OF B-SUBTILIS CHROMOSOME .1. GENETIC STRUCTURE AND REPLICATION ORDER
HARA, H
YOSHIKAW.H
JAPANESE JOURNAL OF GENETICS, 1972, 47 (05): : 345 - 345
[9] In Vivo Assembly and Arrangement of the DNA Translocase SpoIIIE During Chromosome Segregation and Membrane Fission in B. Subtilis
Shin, Jae Yen
Diaz, Cesar
Lopez, Javier
Schnitzbauer, Joerg
Pogliano, Kit
Bustamante, Carlos
BIOPHYSICAL JOURNAL, 2014, 106 (02) : 226A - 226A
[10] Bipolar localization of the replication origin regions of chromosomes in vegetative and sporulating cells of B-subtilis
Webb, CD
Teleman, A
Gordon, S
Straight, A
Belmont, A
Lin, DCH
Grossman, AD
Wright, A
Losick, R
CELL, 1997, 88 (05) : 667 - 674

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