Inhibitory effects of Leuconostoc mesenteroides 1RM3 isolated from narezushi on lipopolysaccharide-induced inflammation in RAW264.7 mouse macrophage cells and dextran sodium sulphate-induced inflammatory bowel disease in mice

The anti-inflammatory bowel disease (anti-IBD) activity of Leuconostoc mesenteroides 1RM3 isolated from saba-narezushi, which has known antioxidant properties, was examined by monitoring its immune and anti-inflammatory activities on murine macrophage RAW264.7 and dextran sodium sulphate (DSS)-induced IBD model mice. Heat-killed Ln. mesenteroides 1RM3 cells promoted nitric oxide (NO) generation in intact RAW264.7 cells. Ln. mesenteroides 1RM3 cells suppressed Escherichia coli 0111 lipopolysaccharide (LPS)-induced NO generation. This bacterium also significantly protected RAW264.7 cells from cytotoxicity due to exposure to H2O2. In the case of mice administered with drinking water containing 5% (w/v) DSS, Ln. mesenteroides 1RM3 (about 9.7 log CFU/ml) suppressed colon inflammation, atrophy, diarrheal and bloody bowl discharge. These results suggest that Ln. mesenteroides 1RM3 could be used as probiotics to improve IBD. (C) 2013 Elsevier Ltd. All rights reserved.