Synchrotron X-ray micro-computed tomography as a tool for in situ elucidation of insect bacteriomes

被引:2
|
作者
Weintraub, P. G. [1 ]
Hoch, H. [2 ]
Muehlethaler, R. [2 ]
Zchori-Fein, E. [3 ]
机构
[1] Gilat Res Ctr, Agr Res Org, IL-85280 Dn Negev, Israel
[2] Humboldt Univ, Museum Nat Kunde, Leibniz Inst Res Evolut & Biodivers, D-10115 Berlin, Germany
[3] Newe Yaar Res Ctr, Agr Res Org, Ramat Yishay, Israel
关键词
Cicadellidae; FISH; Leafhopper; Orosius albicinctus; Symbiotic bacteria; HEMIPTERA CICADELLIDAE; SYMBIOSIS; ENDOSYMBIONTS; PHYTOPLASMAS; DISEASE;
D O I
10.1016/j.asd.2013.11.002
中图分类号
Q96 [昆虫学];
学科分类号
摘要
Obligate bacterial endosymbionts are common, influential associates of arthropods, and are often found in specific organs termed bacteriomes. Three dimensional images of bacteriomes of the leafhopper Orosius albicinctus (Hemiptera: Cicadellidae) were reconstructed from synchrotron-based X-ray microcomputed tomography (CT). Results show that bilateral bacteriomes are located between the first and second abdominal tergites, are mushroom-shaped and consist two different types of tissue. Fluorescence in situ hybridization reveals that the primary bacterial symbiont Sulcia muelleri is in the 'cap' part of the of organ. The technique allows a noninvasive, in situ, means of visualizing bacteriomes and will facilitate understanding their form and function. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:183 / 186
页数:4
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