Phylogenetic analysis of Polymyxa species based on nuclear 5.8S and internal transcribed spacers ribosomal DNA sequences

被引:37
|
作者
Legrève, A
Delfosse, P
Maraite, H
机构
[1] Catholic Univ Louvain, Unite Phytopathol, B-1348 Louvain, Belgium
[2] Int Crops Res Inst Semi Arid Trop, Niamey, Niger
来源
MYCOLOGICAL RESEARCH | 2002年 / 106卷
关键词
D O I
10.1017/S0953756201005391
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A region of the nuclear ribosomal DNA containing the internal transcribed spacer 1 (ITS1), the 5.8S DNA and the internal transcribed spacer 2 (ITS2) was sequenced in 12 Polymyxa graminis and P. betae isolates, with particular emphasis on P. graminis from peanut clump-infested areas of the Indian subcontinent and West-Africa. Four different sequences were obtained from the seven isolates on sorghum or pearl millet, which differed from four sequences previously published for Polymyxa species and obtained for P. graminis isolates on barley, oat and wheat originating from temperate areas in Europe and America (two distinct sequences), for isolates on rice from Colombia and for P. betae isolates on sugar beet from several origins. The sequence variations concerned mainly the composition and length of ITS1 and ITS2 regions. Phylogenetic trees built with the distinct sequences currently known for Polymyxa spp. using parsimony, maximum likeihood and neighbour-joining methods separated P. betae from P. graminis, Within P. graminis, the hierarchy of the clustering partially matched the host range and geographical origin of the isolates. These results confirm the great diversity within P. graminis that has already been observed in host range and temperature requirements studies, and provide new arguments for considering several taxa within the species. On the basis of the ecological requirements and rDNA sequences of distinct P. graminis isolates, five special forms are proposed: P. graminis f. sp. temperata, P. graminis f. sp. tepida, P. graminis f. sp. subtropicalis, P. graminis f. sp. tropicalis and P. graminis f. sp. colombiana.
引用
收藏
页码:138 / 147
页数:10
相关论文
共 50 条
  • [1] Molecular phylogeny of the Aquilegia group (Ranunculaceae) based on internal transcribed spacers and 5.8S nuclear ribosomal DNA
    Ro, KE
    McPheron, BA
    [J]. BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 1997, 25 (05) : 445 - 461
  • [2] A phylogenetic analysis of the Illiciaceae based on sequences of internal transcribed spacers (ITS) of nuclear ribosomal DNA
    Gang Hao
    Richard M. K. Saunders
    Mee-Len Chye
    [J]. Plant Systematics and Evolution, 2000, 223 : 81 - 90
  • [3] A phylogenetic analysis of the Illiciaceae based on sequences of internal transcribed spacers (ITS) of nuclear ribosomal DNA
    Hao, G
    Saunders, RMK
    Chye, ML
    [J]. PLANT SYSTEMATICS AND EVOLUTION, 2000, 223 (1-2) : 81 - 90
  • [4] Analysis of Alexandrium (dinophyceae) species using sequences of the 5.8S ribosomal DNA and internal transcribed spacer regions
    Adachi, M
    Sako, Y
    Ishida, Y
    [J]. JOURNAL OF PHYCOLOGY, 1996, 32 (03) : 424 - 432
  • [5] Phylogenetic analysis of Japanese Rosa species using DNA sequences of nuclear ribosomal internal transcribed spacers (ITS)
    Wu, S
    Ueda, Y
    Nishihara, S
    Matsumoto, S
    [J]. JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY, 2001, 76 (02): : 127 - 132
  • [6] Identification of species of the genus Candida by analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers
    Rosa de Llanos Frutos
    M. Teresa Fernández-Espinar
    Amparo Querol
    [J]. Antonie van Leeuwenhoek, 2004, 85 : 175 - 185
  • [7] Identification of species of the genus Candida by analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers
    Frutos, RD
    Fernández-Espinar, MT
    Querol, A
    [J]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 2004, 85 (03): : 175 - 185
  • [8] Phylogenetic analysis of Kengyilia species based on nuclear ribosomal DNA internal transcribed spacer sequences
    Zeng, J.
    Zhang, L.
    Fan, X.
    Zhang, H. Q.
    Yang, R. W.
    Zhou, Y. H.
    [J]. BIOLOGIA PLANTARUM, 2008, 52 (02) : 231 - 236
  • [9] Phylogenetic analysis of Trichaptum based on nuclear 18S, 5.8S and ITS ribosomal DNA sequences
    Ko, KS
    Hong, SG
    Jung, HS
    [J]. MYCOLOGIA, 1997, 89 (05) : 727 - 734
  • [10] Nucleotide sequence of the internal transcribed spacers and 5.8S region of ribosomal DNA in Pinus pinea L
    Marrocco, R
    Gelati, MT
    Maggini, F
    [J]. DNA SEQUENCE, 1996, 6 (03): : 175 - 177