Community of arbuscular mycorrhizal fungi in drought-resistant plants, Moringa spp., in semiarid regions in Madagascar and Uganda

被引：23

作者：

Yamato, Masahide ^{[1
]}

Ikeda, Shiho ^{[2
]}

Iwase, Koji ^{[3
]}

机构：

[1] Gen Environm Technos Co Ltd, Dept Environm, Chuou Ku, Osaka 5410052, Japan

[2] Forest Dev Technol Inst, Kyoto, Japan

[3] Tottori Univ, Fac Agr, Fungus Mushroom Resource & Res Ctr, Tottori 680, Japan

来源：

MYCOSCIENCE | 2009年 / 50卷 / 02期

关键词：

Arbuscular mycorrhizal fungi; Glomus intraradices; Moringa drouhardii; Moringa hildebrandtii; Moringa oleifera; MOLECULAR DIVERSITY; WESTERN-GHATS; TOLERANCE; PATTERNS; GLOMUS;

D O I：

10.1007/s10267-008-0459-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The community structure of arbuscular mycorrhizal (AM) fungi in the roots of drought-resistant trees, Moringa spp., was examined in semiarid regions in Madagascar and Uganda. Root samples were collected from 8 individuals of M. hildebrandtii and 2 individuals of M. drouhardii in Madagascar and from 21 individuals of M. oleifera in Uganda. Total DNA was extracted from the root samples, and partial nSSU rDNA of AM fungi was amplified using a universal eukaryotic primer NS31 and an AM fungalspecific primer AM1. The PCR products were cloned and divided by restriction fragment length polymorphism (RFLP) analysis with HinfI and RsaI. Some representatives in each RFLP types were sequenced, and a neighbor-joining phylogenetic analysis was conducted for the obtained sequences with analogous sequences of AM fungi. The RFLP and phylogenetic analyses showed that AM fungi closely related to Glomus intraradices or G. sinuosum were detected in many samples. The AM fungal groups frequently detected in the Moringa spp. might be widely distributed species in semiarid environments.

引用

页码：100 / 105

页数：6

共 3 条

[1] Interaction between arbuscular mycorrhizal fungi and Bacillus spp. In soil enhancing growth of crop plants
Nanjundappa A.
Bagyaraj D.J.
Saxena A.K.
Kumar M.
Chakdar H.
[J]. Fungal Biology and Biotechnology, 6 (1)
[2] The detection of Glomus spp. (arbuscular mycorrhizal fungi) forming mycorrhizas in three plants, at different stages of seedling development, using mycorrhiza-specific isozymes
Tisserant, B
Brenac, V
Requena, N
Jeffries, P
Dodd, JC
[J]. NEW PHYTOLOGIST, 1998, 138 (02) : 225 - 239
[3] Contrasting the Community Structure of Arbuscular Mycorrhizal Fungi from Hydrocarbon-Contaminated and Uncontaminated Soils following Willow (Salix spp. L.) Planting
Hassan, Saad El-Din
Bell, Terrence H.
Stefani, Franck O. P.
Denis, David
Hijri, Mohamed
St-Arnaud, Marc
[J]. PLOS ONE, 2014, 9 (07):

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