Comparison of animal and plant DNA-adducts after exposure to benzo(a)pyrene.

被引:4
|
作者
PfohlLeszkowicz, A
Masfaraud, JF
WeberLotfi, F
Marty, J
Guillemaut, P
Castegnaro, M
机构
[1] ENSAT,LAB TOXICOL & SECUR ALIMENTAIRE,F-31076 TOULOUSE,FRANCE
[2] CTR ENVIRONM SCI,F-57040 METZ,FRANCE
[3] IUT LOUIS PASTEUR,LAB BIOL VEGETALE,F-67000 STRASBOURG,FRANCE
[4] UNIV TOULOUSE 3,CTR DEV BIOL,F-31062 TOULOUSE,FRANCE
[5] INT AGCY RES CANC,F-69008 LYON,FRANCE
关键词
DNA-adducts; P-32-postlabelling; biotransformation; benzo(a)pyrene; biomarkers;
D O I
10.1080/10406639608034713
中图分类号
O62 [有机化学];
学科分类号
070303 ; 081704 ;
摘要
The occurrence of abnormal hypermodified nucleotides on the DNA upon xenobiotic exposure has long been considered as a characteristic of carcinogenesis and mutagenesis in animal cells. We have previously shown that DNA adducts could also be formed in plants exposed to xenobiotics in natural and controlled conditions. In this study we have compared the DNA adducts formed in different animal species and in different plants after benzo(a)pyrene (B(a)P) exposure. The main DNA adduct in mice stomach and skin correspond to the 7,8-diol 9,10-epoxide B(a)P-guanine. In liver from rat, fish and Xenopus, this adduct is detected, but is not the major one. In plants analyzed, this adduct is never formed. Ten different adducts are detected in plants. This result indicates that the metabolic pathway leading to genotoxic metabolites is different with species. In conclusion, the result suggest that risk assessment for human and environment due to genotoxic compounds should be realized using multiple species assays.
引用
收藏
页码:323 / 331
页数:9
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