Cloning and characterization of extracellular metal protease gene of the algicidal marine bacterium Pseudoalteromonas sp strain A28

被引：35

作者：

Lee, SO ^{[1
]}

Kato, J ^{[1
]}

Nakashima, K ^{[1
]}

Kuroda, A ^{[1
]}

Ikeda, T ^{[1
]}

Takiguchi, N ^{[1
]}

Ohtake, H ^{[1
]}

机构：

[1] Hiroshima Univ, Dept Mol Biotechnol, Grad Sch Adv Sci Matter, Higashihiroshima, Hiroshima 7398530, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2002年 / 66卷 / 06期

关键词：

Pseudoalteromonas; algicidal marine bacterium; extracellular metal protease; red tide algae;

D O I：

10.1271/bbb.66.1366

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene (empI) encoding an extracellular metal protease was isolated from a Pseudoalteromonas sp. strain A28 DNA library. The recombinant EmpI protein was expressed in E. coli and purified. Paper-disk assays showed that the purified protease had potent algicidal activity. A skim milk-polyacrylamide gel electrophoresis protease assay showed that the 38-kDa band of protease activity, which co-migrated with purified EmpI and was sensitive to 1,10-phenathroline, was detected in the extracellular supernatant of A28.

引用

页码：1366 / 1369

页数：4

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