Cholesterol depletion induces ANTXR2-dependent activation of MMP-2 via ERK1/2 phosphorylation in neuroglioma U251 cell

被引：19
|
作者：
Zou, Jianwen [1 ]
Xu, Li [2 ]
Ju, Ying [1 ]
Zhang, Peili [1 ]
Wang, Yong [1 ]
Zhang, Bingchang [1 ]
机构：
[1] Shandong Univ, Shandong Prov Hosp, Dept Clin Lab, Jinan 250021, Shandong, Peoples R China
[2] Zaozhuang Municipal Hosp, Dept Clin Lab, Zaozhuang 277100, Shandong, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2014年 / 452卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Cholesterol; ANTXR2; ERK phosphorylation; MT1-MMP; MMP-2; CAPILLARY MORPHOGENESIS PROTEIN-2; LIPID RAFTS; MEMBRANE; BINDING; CAVEOLAE; COLLAGEN;
D O I：
10.1016/j.bbrc.2014.06.001
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Cholesterol is a critical component of lipid rafts implicated in regulating multiple signal transduction. The anthrax toxin receptor 2 (ANTXR2) is a type I membrane protein acting as the second receptor for the anthrax toxin. In this study, we first investigated the association between cholesterol and ANTXR2. We provided the evidence that cholesterol depletion by methyl-beta-cyclodextrin (M beta CD) promoted ANTXR2 expression in U251 neuroglioma cell, which was reversed by cholesterol supplement. M beta CD-induced ANTXR2 up-regulation contributed to ERK1/2 phosphorylation, which was responsible for MT1-MMP and MMP-2 activation. Our data suggested that cellular cholesterol regulated ANTXR2-dependent activation of MMP-2 via ERK1/2 phosphorylation in neuroglioma U251 cell. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码：186 / 190
页数：5
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