Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C1

被引：66
|
作者：
Huang, Yukun [1 ]
Chen, Xiujuan [1 ]
Duan, Nuo [1 ]
Wu, Shijia [1 ]
Wang, Zhouping [1 ]
Wei, Xinlin [2 ]
Wang, Yuanfeng [2 ]
机构：
[1] Jiangnan Univ, Sch Food Sci & Technol, Synerget Innovat Ctr Food Safety & Nutr, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China
[2] Shanghai Normal Univ, Collage Life & Environm Sci, Shanghai 200234, Peoples R China
来源：
FOOD CHEMISTRY | 2015年 / 166卷
关键词：
Aptamer; Staphylococcus aureus enterotoxin C1; SELEX; Graphene oxide; Food; IN-VITRO SELECTION; GRAPHENE OXIDE; PCR PRIMERS; NANOPARTICLES; IDENTIFICATION; BINDING; SELEX; C2;
D O I：
10.1016/j.foodchem.2014.06.039
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Enterotoxins from pathogenic bacteria are known as the main reason that can cause the bacterial foodborne diseases. In this study, aptamers that bound to Staphylococcus aureus enterotoxin C1 (SEC1) with high affinity and selectivity were generated in vitro by twelve rounds of selection based on magnetic separation technology, with a low-level dissociation constant (K-d) value of 65.14 +/- 11.64 nmol/L of aptamer C10. Aptamer-based quantification of SEC1 in the food sample by a graphene oxide (GO)-based method was implemented to investigate the potential of the aptamer against SEC1 with a limit of detection of 6 ng/mL. On the basis of this work, biosensors using the selected SEC1 aptamers as new molecular recognition elements could be applied for innovative determinations of SEC1. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码：623 / 629
页数：7
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