Zinc ions inhibit the protein-DNA complex formation between cyanobacterial transcription factor SmtB and its recognition DNA sequences

被引：3

作者：

Morita, EH

Wakamatsu, M

Uegaki, K

Yumoto, N

Kyogoku, Y

Hayashi, H

机构：

[1] Ehime Univ, Fac Sci, Dept Chem, Matsuyama, Ehime 7908577, Japan

[2] Ehime Univ, Satellite Venture Business Lab, Matsuyama, Ehime 7908577, Japan

[3] Ehime Univ, Ctr Gene Res, Matsuyama, Ehime 7908566, Japan

[4] Ehime Univ, Grad Sch Sci & Engn, Matsuyama, Ehime 7908577, Japan

[5] Natl Inst Adv Ind Sci & Technol, Ikeda, Osaka 5638577, Japan

[6] Natl Inst Adv Ind Sci & Technol Kansai, Tokyo 5638577, Japan

[7] Natl Inst Adv Ind Sci & Technol, Biol Informat Res Ctr, Koto Ku, Tokyo 1350064, Japan

来源：

PLANT AND CELL PHYSIOLOGY | 2002年 / 43卷 / 10期

关键词：

cyanobacteria; heavy-metal ion sensor; operator/promoter region; transcription factor;

D O I：

10.1093/pcp/pcf140

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

SmtB is a trans-acting dimeric repressor of the metal-regulated smtA gene, and the release of SmtB from the smtA operator/promoter region is essential for the tolerance to Zn2+ stress by SmtA expression. Gel retardation assaying demonstrated that different sizes of SmtB-DNA complexes were formed depending on the DNA sequences, and the amounts of these complexes decreased in the presence of Zn2+. Here, we present the first direct evidence that Zn2+ (>4 muM) inhibits the SmtB-DNA complex formation in vitro, which ensures the physiological functions of SmtB as a Zn2+ sensor and a transcription factor.

引用

页码：1254 / 1258

页数：5

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