Direct detection of bacterial faecal indicators in water samples using PCR

被引:16
|
作者
Horakova, K. [1 ]
Mlejnkova, H.
Mlejnek, P.
机构
[1] Masaryk Univ, Fac Sci, Dept Microbiol, CS-61137 Brno, Czech Republic
[2] TG Masaryk Water Res Inst, Brno, Czech Republic
[3] Palacky Univ, Fac Med, Dept Biol, CR-77147 Olomouc, Czech Republic
关键词
coliform bacteria; Escherichia coli; faecal indicator; PCR;
D O I
10.2166/wst.2006.459
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The presence of enteric pathogens in water resources represents a serious risk for public health. Therefore, their precise detection, and especially detection of E coli, which is obviously regarded as the main indicator of faecal contamination of water, is an essential step in ensuring bacterial safety of water. Numerous PCR protocols for detection of E coli have been published to date. They are usually based on amplification of regions derived from lacZ (beta-D-galactosidase) and uidA (beta-D-glucuronidase) gene sequences. However, these methods are not universal enough for precise detection of all E coli strains found in water samples. We developed a novel triplex PCR method for detection of E coli in which cyd gene coding for cytochrome bd complex was co-amplified along with lacZ and uidA genes. Our triplex PCR approach significantly increases the specificity and reliability of E coli detection in water samples. This approach allowed us to distinguish Shigella flexneri from E coli. In addition, we were able to detect even non-coliform Klebsiella and Raoutella spp., some of which can also cause infections to humans.
引用
收藏
页码:135 / 140
页数:6
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