Over-expression of the Arabidopsis proton-pyrophosphatase AVP1 enhances transplant survival, root mass, and fruit development under limiting phosphorus conditions

被引:48
|
作者
Yang, Haibing [1 ]
Zhang, Xiao [1 ,2 ]
Gaxiola, Roberto A. [3 ]
Xu, Guohua [2 ]
Peer, Wendy Ann [1 ,4 ,5 ]
Murphy, Angus S. [1 ,5 ]
机构
[1] Purdue Univ, Dept Hort, W Lafayette, IN 47907 USA
[2] Nanjing Agr Univ, Coll Resources & Environm Sci, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China
[3] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA
[4] Univ Maryland, Dept Environm Sci & Technol, College Pk, MD 20742 USA
[5] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA
基金
美国国家科学基金会;
关键词
Fruit development; H+-pyrophosphatase; phosphorus; root development; tomato; transplant efficiency; H+-PPASE AVP1; PLASMA-MEMBRANE; SALT TOLERANCE; UP-REGULATION; PLANTS; OVEREXPRESSION; TRANSPORT; IMPROVES; GENE;
D O I
10.1093/jxb/eru149
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Phosphorus (P), an element required for plant growth, fruit set, fruit development, and fruit ripening, can be deficient or unavailable in agricultural soils. Previously, it was shown that over-expression of a proton-pyrophosphatase gene AVP1/AVP1D (AVP1DOX) in Arabidopsis, rice, and tomato resulted in the enhancement of root branching and overall mass with the result of increased mineral P acquisition. However, although AVP1 over-expression also increased shoot biomass in Arabidopsis, this effect was not observed in tomato under phosphate-sufficient conditions. AVP1DOX tomato plants exhibited increased rootward auxin transport and root acidification compared with control plants. AVP1DOX tomato plants were analysed in detail under limiting P conditions in greenhouse and field trials. AVP1DOX plants produced 25% (P=0.001) more marketable ripened fruit per plant under P-deficient conditions compared with the controls. Further, under low phosphate conditions, AVP1DOX plants displayed increased phosphate transport from leaf (source) to fruit (sink) compared to controls. AVP1DOX plants also showed an 11% increase in transplant survival (P<0.01) in both greenhouse and field trials compared with the control plants. These results suggest that selection of tomato cultivars for increased proton pyrophosphatase gene expression could be useful when selecting for cultivars to be grown on marginal soils.
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页码:3045 / 3053
页数:9
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