A sensitive LC-MS/MS method for the quantitative determination of biflorin in rat plasma and its application to pharmacokinetic studies

被引:8
|
作者
Yang, Seung Jun [1 ]
Ryu, Jong Hoon [2 ]
Jang, Dae Sik [3 ]
Yang, Liang [1 ]
Han, Hyo-Kyung [1 ]
机构
[1] Dongguk Univ Seoul, Coll Pharm, BK21 Plus Project Team, Goyang, South Korea
[2] Kyung Hee Univ, Coll Pharm, Seoul, South Korea
[3] Kyung Hee Univ, Dept Life & Nanopharmaceut Sci, Seoul, South Korea
基金
新加坡国家研究基金会;
关键词
Biflorin; LC-MS/MS; Rat; Plasma; Pharmacokinetics; CHROMONE-C-GLUCOSIDE; CONSTITUENTS; NARINGIN;
D O I
10.1016/j.jpba.2015.07.032
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A rapid, sensitive and selective liquid chromatography-tandem mass spectrometric method (LC-MS/MS) was developed for the quantification of biflorin in rat plasma. Using naringin as an internal standard, plasma samples were subjected to a direct protein precipitation process using methanol. Chromatographic separation was achieved on a Gemini C18 column with an isocratic mobile phase consisting of 0.1% formic acid and methanol (50:50, v/v) at a flow rate of 0.5 mL/min. Biflorin was analyzed in the multiple reaction monitoring mode with negative electrospray ionization. The precursor/product ion pairs were m/z 353.0/205.0 and m/z 579.0/271.0 for biflorin and the IS, respectively. The calibration curve was linear over the concentration range of 5-2000 ng/mL. The intra- and inter-day precision was less than 7.3% and the accuracy ranged from 96.5 to 103.3%. No significant variation was observed in the stability tests. This method was successfully applied to a pharmacokinetic study of biflorin after the intravenous and oral administration of biflorin to rats. The half-life and oral bioavailability of biflorin were determined as 3.4 h and 43%, respectively. This is the first report on the quantitative determination of biflorin in rat plasma as well as the pharmacokinetic characterization of biflorin, which should provide a meaningful foundation for further preclinical and clinical applications of biflorin. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:272 / 276
页数:5
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