Retinal safety evaluation of two-photon laser scanning in rats

被引：4

作者：

Jayabalan, Gopal Swamy ^{[1
,2
]}

Bille, Josef F. ^{[1
]}

Mao, Xiao Wen ^{[3
]}

Gimbel, Howard, V ^{[2
]}

Rauser, Michael E. ^{[2
]}

Wenz, Frederik ^{[1
]}

Fan, Joseph T. ^{[2
,3
]}

机构：

[1] Heidelberg Univ, Med Fac Mannheim, Clin Radiotherapy & Radiat Oncol, D-68167 Mannheim, Germany

[2] Loma Linda Univ, Eye Inst, Loma Linda, CA 92354 USA

[3] Loma Linda Univ, Dept Basic Sci, Loma Linda, CA 92350 USA

来源：

BIOMEDICAL OPTICS EXPRESS | 2019年 / 10卷 / 07期

关键词：

ADAPTIVE OPTICS; DAMAGE; MICROSCOPY; EXPOSURES;

D O I：

10.1364/BOE.10.003217

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Safe use of retinal imaging with two-photon excitation in human eyes is crucial, as the effects of ultrashort pulsed lasers on the retina are relatively unknown. At the time of the study, the laser safety standards Were inadequate due to the lack of biological data. This article addresses the feasibility of two-photon retinal imaging with respect to laser safety. In this stud), rat retinas Were evaluated at various laser exposure levels and with different laser parameters to determine the effects of laser-induced optical damage. The results Were experimentally verified using confocal reflectance imaging, two-photon fluorescein angiography, immunohistochemistry, and correlated to the IEC 60825-1 laser safety standard. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

引用

页码：3217 / 3231

页数：15

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