Assay of soluble guanylate cyclase activity by isocratic high-performance liquid chromatography

被引：11
|
作者：
Pietta, PG [1 ]
Mauri, PL [1 ]
Gardana, C [1 ]
Benazzi, L [1 ]
机构：
[1] DIPARTIMENTO SCI & TECNOL BIOMED, I-20133 MILAN, ITALY
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 1997年 / 690卷 / 1-2期
关键词：
guanylate cyclase; enzymes;
D O I：
10.1016/S0378-4347(96)00414-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A HPLC method alternative to labelled or unlabelled procedures was developed for the assay of guanylate cyclase (GC) activity. The substrate (GTP) and the product (cGMP) of the enzymatic re;action were separated in the isocratic mode on a mu Bondapak C-18 column. The activity of GC was linearly dependent on the amount of cGMP produced in the presence of sodium nitroprusside. This approach was applied to follow the purification of GC from bovine lung and to evaluate its stability in different storage conditions.
引用
收藏
页码：343 / 347
页数：5
相关论文
共 50 条
  • [1] Assay of human platelet guanylate cyclase activity by high-performance liquid chromatography with fluorescence derivatization
    Soda, K
    Ohba, Y
    Zaitsu, K
    [J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2001, 752 (01): : 55 - 60
  • [2] Assay of adenylyl cyclase activity by ion-exchange high-performance liquid chromatography
    Liao, F
    Zhang, XP
    Zhou, QX
    Zeng, ZC
    Chen, WY
    [J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA, 2000, 32 (06): : 661 - 664
  • [3] A reverse-phase, isocratic high-performance liquid chromatography assay for levofloxacin
    Tobin, CM
    Sunderland, J
    White, LO
    MacGowan, AP
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1999, 43 (03) : 434 - 435
  • [4] A simple, isocratic high-performance liquid chromatography assay for linezolid in human serum
    Tobin, CM
    Sunderland, J
    White, LO
    MacGowan, AP
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2001, 48 (05) : 605 - 608
  • [5] GLYCOSYLATED HEMOGLOBIN DETERMINATIONS BY ISOCRATIC HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    DAVID, RM
    SHIHABI, ZK
    [J]. JOURNAL OF LIQUID CHROMATOGRAPHY, 1984, 7 (14): : 2875 - 2885
  • [6] ISOCRATIC HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF BILE-PIGMENTS
    COLE, KD
    LITTLE, GH
    [J]. JOURNAL OF CHROMATOGRAPHY, 1982, 227 (02): : 503 - 509
  • [7] ISOCRATIC SEPARATION OF FOOD FOLACIN BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    BRIGGS, DR
    JONES, GP
    SAEEUNG, P
    [J]. JOURNAL OF CHROMATOGRAPHY, 1982, 246 (01): : 165 - 168
  • [8] Assay of nicotinamide deamidase activity using high-performance liquid chromatography
    Oishi, M
    Ogasawara, Y
    Ishii, K
    Tanabe, S
    [J]. JOURNAL OF CHROMATOGRAPHY B, 1998, 720 (1-2): : 59 - 64
  • [9] ASSAY OF CHALCONE SYNTHASE ACTIVITY BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    ZUURBIER, KWM
    FUNG, SY
    SCHEFFER, JJC
    VERPOORTE, R
    [J]. PHYTOCHEMISTRY, 1993, 34 (05) : 1225 - 1229
  • [10] THE ASSAY OF CHLORAMPHENICOL ACETYLTRANSFERASE ACTIVITY BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    LOVERING, AM
    WHITE, LO
    REEVES, DS
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1986, 17 (06) : 821 - 825
12345