Penicillinase-based amperometric biosensor for penicillin G

被引：40
|
作者：
Goncalves, Luis Moreira [1 ]
Callera, Welder F. A. [2 ]
Sotomayor, Maria D. P. T. [2 ]
Bueno, Paulo R. [2 ]
机构：
[1] Univ Porto, Fac Ciencias, Dept Quim & Bioquim, REQUIMTE, P-4169007 Oporto, Portugal
[2] Univ Estadual Paulista, Inst Quim, BR-14800900 Sao Paulo, Brazil
来源：
ELECTROCHEMISTRY COMMUNICATIONS | 2014年 / 38卷
基金：
巴西圣保罗研究基金会;
关键词：
Cysteine; Metallo-beta-lactamase; Self-assembled monolayer (SAM); beta-Lactam antibiotic; Lineweaver-Burk plot; QUARTZ-CRYSTAL MICROBALANCE; BETA-LACTAMASE; ENZYME; EQUATIONS; KINETICS; GOLD; PH;
D O I：
10.1016/j.elecom.2013.11.022
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
A biosensor for penicillin G was created by immobilizing penicillinase to a gold electrode by means of a cysteine self-assembled monolayer. The biosensor amperometrically monitored the catalytic hydrolysis of penicillin in a very sensible manner. Furthermore, it was successfully used to measure the Michaelis-Menten enzymatic constant and a low limit of detection of 4.5 nM was obtained. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：131 / 133
页数：3
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