The non-psychoactive phytocannabinoid cannabidiol (CBD) attenuates pro-inflammatory mediators, T cell infiltration, and thermal sensitivity following spinal cord injury in mice

被引：52

作者：

Li, Hongbo ^{[1
]}

Kong, Weimin ^{[2
]}

Chambers, Christina R. ^{[1
]}

Yu, Daohai ^{[3
]}

Ganea, Doina ^{[2
]}

Tuma, Ronald F. ^{[4
]}

Ward, Sara Jane ^{[5
]}

机构：

[1] Temple Univ, Lewis Katz Sch Med, Ctr Subst Abuse Res, Philadelphia, PA 19140 USA

[2] Temple Univ, Lewis Katz Sch Med, Microbiol & Immunol Dept, Philadelphia, PA 19140 USA

[3] Temple Univ, Lewis Katz Sch Med, Dept Clin Sci, Philadelphia, PA 19140 USA

[4] Temple Univ, Lewis Katz Sch Med, Ctr Subst Abuse Res, Dept Physiol, Philadelphia, PA 19140 USA

[5] Temple Univ, Lewis Katz Sch Med, Ctr Subst Abuse Res, Dept Pharmacol, Philadelphia, PA 19140 USA

来源：

CELLULAR IMMUNOLOGY | 2018年 / 329卷

关键词：

Spinal cord injury; Neuropathic pain; Cannabidiol; Inflammation; T cell; NEUROPATHIC PAIN; MECHANICAL ALLODYNIA; ACTIVE IMMUNIZATION; 5-HT1A RECEPTORS; RAT PAW; METHYLPREDNISOLONE; CONTUSION; MICROGLIA; RECOVERY; MAINTENANCE;

D O I：

10.1016/j.cellimm.2018.02.016

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We evaluated the effects of the non-psychoactive cannabinoid cannabidiol (CBD) on the inflammatory response and recovery of function following spinal cord injury (SCI). Female C57Bl/6 mice were exposed to spinal cord contusion injury (T9-10) and received vehicle or CBD (1.5 mg/kg IP) injections for 10 weeks following injury. The effect of SCI and CBD treatment on inflammation was assessed via microarray, qRT-PCR and flow cytometry. Locomotor and bladder function and changes in thermal and mechanical hind paw sensitivity were also evaluated. There was a significant decrease in pro-inflammatory cytokines and chemokines associated with T-cell differentiation and invasion in the SCI-CBD group as well as a decrease in T cell invasion into the injured cord. A higher percentage of SCI mice in the vehicle-treated group (SCI-VEH) went on to develop moderate to severe (0-65.9% baseline thermal threshold) thermal sensitivity as compared with CBD-treated (SCI-CBD) mice. CBD did not affect recovery of locomotor or bladder function following SCI. Taken together, CBD treatment attenuated the development of thermal sensitivity following spinal cord injury and this effect may be related to protection against pathological T-cell invasion.

引用

页码：1 / 9

页数：9