The aim of the present study was to survey the influence of municipal sewage irrigation on the farmland soil and provide theoretical basis for it. Two field trials were carried out at Xuzhou of Jiangsu Province, China. Microbial cultivation experimental method and irrigation with tap water as contrast treatment were used to evaluate effects of municipal sewage irrigation on farmland soil properties and microorganisms quantity. The results indicated that the influence of sewage irrigation on the total nitrogen, phosphorus of soil is not obvious, but ammonia nitrogen is lower, available phosphorous and organic matter is higher than the contrast treatment. The nitrite bacteria, nitrate bacteria and denitrifying bacteria quantity are reduced, but the ammonia oxidizing bacteria, aerobic nitrogens-fixing bacteria and cellulose decomposing bacteria quantity are increased after sewage irrigation. And correlation analysis showed that the quantity of some microorganisms is closelier related to ammonia nitrogen, available phosphorous and organic matter content than the contrast treatment.