Dual Spinneret Electrospun Polyurethane/PVA-Gelatin Nanofibrous Scaffolds Containing Cinnamon Essential Oil and Nanoceria for Chronic Diabetic Wound Healing: Preparation, Physicochemical Characterization and In-Vitro Evaluation

被引：25

作者：

Hussein, Mohamed Ahmed Mohamady ^{[1
,2
]}

Gunduz, Oguzhan ^{[3
,4
]}

Sahin, Ali ^{[5
,6
]}

Grinholc, Mariusz ^{[7
]}

El-Sherbiny, Ibrahim Mohamed ^{[8
]}

Megahed, Mosaad ^{[1
]}

机构：

[1] Univ Hosp RWTH Aachen, Clin Dermatol, D-52074 Aachen, Germany

[2] Natl Res Ctr, Dept Pharmacol, Med Res Div, Cairo 12622, Egypt

[3] Marmara Univ, Ctr Nanotechnol & Biomat Applicat & Res NBUAM, TR-34722 Istanbul, Turkey

[4] Marmara Univ, Fac Technol, Dept Met & Mat Engn, TR-34722 Istanbul, Turkey

[5] Marmara Univ, Sch Med, Dept Biochem, TR-34854 Istanbul, Turkey

[6] Marmara Univ, Genet & Metab Dis Res & Invest Ctr GEMHAM, TR-34854 Istanbul, Turkey

[7] Univ Gdansk, Intercollegiate Fac Biotechnol, Dept Biotechnol, Lab Mol Diagnost, PL-80307 Gdansk, Poland

[8] Zewail City Sci & Technol, Nanomed Lab, Ctr Mat Sci CMS, Giza 12578, Egypt

来源：

MOLECULES | 2022年 / 27卷 / 07期

关键词：

nanoceria; dual spinneret electrospinning; PU; PVA-gelatin-based nanofibrous scaffolds; diabetic wound healing; ANTIMICROBIAL PROPERTIES; ANTIBACTERIAL ACTIVITY; COMPOSITE NANOFIBERS; NANOPARTICLES; OXIDE; FILMS; MECHANISM; MEMBRANES; COMBINATION; DRESSINGS;

D O I：

10.3390/molecules27072146

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, a dual spinneret electrospinning technique was applied to fabricate a series of polyurethane (PU) and polyvinyl alcohol-gelatin (PVA/Gel) nanofibrous scaffolds. The study aims to enhance the properties of PU/PVA-Gel NFs loaded with a low dose of nanoceria through the incorporation of cinnamon essential oil (CEO). The as-prepared nCeO(2) were embedded into the PVA/Gel nanofibrous layer, where the cinnamon essential oil (CEO) was incorporated into the PU nanofibrous layer. The morphology, thermal stability, mechanical properties, and chemical composition of the produced NF mats were investigated by STEM, DSC, and FTIR. The obtained results showed improvement in the mechanical, and thermal stability of the dual-fiber scaffolds by adding CEO along with nanoceria. The cytotoxicity evaluation revealed that the incorporation of CEO to PU/PVA-Gel loaded with a low dose of nanoceria could enhance the cell population compared to using pure PU/PVA-Gel NFs. Moreover, the presence of CEO could inhibit the growth rate of S. aureus more than E. coli. To our knowledge, this is the first time such nanofibrous membranes composed of PU and PVA-Gel have been produced. The first time was to load the nanofibrous membranes with both CEO and nCeO(2). The obtained results indicate that the proposed PU/PVA-Gel NFs represent promising platforms with CEO and nCeO(2) for effectively managing diabetic wounds.

引用

页数：20