Catalytic mechanism of Kdo8P synthase. Pre-steady-state kinetic analysis using rapid chemical quench flow methods

被引：0
|
作者：
Liang, PH
Kohen, A
Baasov, T
Anderson, KS
机构：
[1] TECHNION ISRAEL INST TECHNOL,DEPT CHEM,IL-32000 HAIFA,ISRAEL
[2] YALE UNIV,SCH MED,DEPT PHARMACOL,NEW HAVEN,CT 06510
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1997年 / 7卷 / 19期
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D O I：
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中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
The catalytic mechanism of 3-deoxy-D-manno-2-octulosonate-8-phosphate (Kdo8P) synthase from Escherichia coli was investigated under pre-steady-state conditions using rapid chemical quench flow methods. The results suggest the formation of acyclic bisphosphate 1 as a reaction intermediate. (C) 1997 Elsevier Science Ltd.
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页码：2463 / 2468
页数：6
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