Minimal genome-wide human CRISPR-Cas9 library

被引：36

作者：

Goncalves, Emanuel ^{[1
]}

Thomas, Mark ^{[1
]}

Behan, Fiona M. ^{[1
]}

Picco, Gabriele ^{[1
]}

Pacini, Clare ^{[1
,2
]}

Allen, Felicity ^{[1
]}

Vinceti, Alessandro ^{[3
]}

Sharma, Mamta ^{[1
]}

Jackson, David A. ^{[1
]}

Price, Stacey ^{[1
]}

Beaver, Charlotte M. ^{[1
]}

Dovey, Oliver ^{[1
]}

Parry-Smith, David ^{[1
]}

Iorio, Francesco ^{[1
,3
]}

Parts, Leopold ^{[1
,4
]}

Yusa, Kosuke ^{[5
]}

Garnett, Mathew J. ^{[1
]}

机构：

[1] Wellcome Sanger Inst, Wellcome Genome Campus, Hinxton, England

[2] Open Targets, Wellcome Genome Campus, Cambridge CB10 1SA, England

[3] Human Technopole, Via Cristina Belgioioso 147, I-20157 Milan, Italy

[4] Univ Tartu, Dept Comp Sci, 18 Narva St, Tartu, Estonia

[5] Kyoto Univ, Inst Frontier Life & Med Sci, Kyoto 6068507, Japan

来源：

GENOME BIOLOGY | 2021年 / 22卷 / 01期

基金：

英国惠康基金;

关键词：

CRISPR-Cas9; Genome-wide; Minimal library; Organoid; KS score; GENETIC SCREENS; DRUG-SENSITIVITY; POOLED LIBRARY; KNOCKOUT; DESIGN; VULNERABILITIES; IDENTIFICATION; SPECIFICITY; DISCOVERY; TARGETS;

D O I：

10.1186/s13059-021-02268-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

CRISPR guide RNA libraries have been iteratively improved to provide increasingly efficient reagents, although their large size is a barrier for many applications. We design an optimised minimal genome-wide human CRISPR-Cas9 library (MinLibCas9) by mining existing large-scale gene loss-of-function datasets, resulting in a greater than 42% reduction in size compared to other CRISPR-Cas9 libraries while preserving assay sensitivity and specificity. MinLibCas9 provides backward compatibility with existing datasets, increases the dynamic range of CRISPR-Cas9 screens and extends their application to complex models and assays.

引用

页数：14

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