New, simple and validated UV-spectrophotometric methods for the estimation of pyridoxine hydrochloride in bulk and formulation

In this study, two rapid, simple and accurate spectrophotometric methods for the determination of pyridoxine hydrochloride, in bulk and in pharmaceutical formulations are described. These methods are based on the reaction of pyridoxine hydrochloride with ferric salts, i.e. ferric nitrate and ferric ammonium citrate. The reaction produces a yellowish orange color which absorbs maximally at 445 and 450 nm for ferric nitrate and ferric ammonium citrate, respectively. Beer's law was obeyed in the range of 5-50 mu g mL(-1) for both reagents. The regression analysis of Beer's plot showed a good correlation coefficient (r(2)= 0.9987, 0.9982). The results were validated analytically and statistically according to International Conference on Harmonization (ICH) guidelines. The proposed methods were applied to the determination of pyridoxine hydrochloride in bulk and pharmaceutical preparations with good results.