Direct electrochemistry of hemoglobin in biomembrane-like DHP-PDDA polyion-surfactant composite films

被引：38
|
作者：
Liu, HY [1 ]
Wang, LW [1 ]
Hu, NF [1 ]
机构：
[1] Beijing Normal Univ, Dept Chem, Beijing 100875, Peoples R China
来源：
ELECTROCHIMICA ACTA | 2002年 / 47卷 / 15期
基金：
中国国家自然科学基金;
关键词：
hemoglobin; dihexadecylphosphate; poly(diallyldimethylammonium); direct electrochemistry; electrocatalysis;
D O I：
10.1016/S0013-4686(02)00128-7
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
Hb-DHP-PDDA films were assembled by incorporating hemoglobin (Hb) into polyion-surfactant DHP-PDDA composite films on pyrolytic graphite (PG) electrodes, where DHP is an anionic surfactant dihexa decylphosphate, and PDDA is a polycation poly(diallyldimethylammonium). Cyclic voltammetry of Hb-DHP-PDDA films showed a pair of stable and reversible peaks for Hb Fe(III)/Fe(II) redox couple at about -0.34 V versus saturated calomel electrode (SCE) in pH 7.0 buffers. The electron transfer rate between Hb and PG electrodes was greatly facilitated in microenvironment of the films. X-ray diffraction (XRD) and differential scanning calorimetry (DSC) suggest that in both DHP-PDDA and Hb-DHP-PDDA films, DHP is self-assembled into an ordered bilayer structure which is sandwiched by PDDA backbone layers. Positions of Soret absorption band indicate that Hb keeps its secondary structure similar to its native state in the films in the medium pH range. Hb could act as an enzymatic catalyst in DHP-PDDA films to catalyze reduction of oxygen, trichloroacetic acid, and nitrite with significant decrease of overpotential. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：2515 / 2523
页数：9
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