Hydrogen peroxide production in cultured RPE cells exposed to near infrared lasers

被引：3
|
作者：
Denton, ML [1 ]
Eikum, DM [1 ]
Stolarski, DJ [1 ]
Noojin, GD [1 ]
Thomas, RJB [1 ]
Glickman, RD [1 ]
Rockwell, BA [1 ]
机构：
[1] Northrop Grumman Informat Technol, San Antonio, TX 78228 USA
来源：
LASER TISSUE INTERACTION XIII: PHOTOCHEMICAL, PHOTOTHERMAL, AND PHOTOMECHANICAL | 2002年 / 4617卷
关键词：
RPE cells; multi-photon; mode-locked; fluorescence microscopy;
D O I：
10.1117/12.472518
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Hydrogen peroxide (H2O2) in situ synthesis within cultures of retinal pigment epithelial cells exposed to near-IR (NIR) continuous wave (CW) and mode-locked lasers is investigated. Any production of H2O2 as a consequence of NIR laser exposure is indicative of a multiphoton absorption event, resulting in an intracellular photochemical reaction. Comparisons of mode-locked and CW laser threshold energies required for the photo-oxidative reaction provide support for an enhancement in multiphoton absorption in tissues exposed to the high peak powers inherent in mode-locked laser beams. Due to different fluorescent staining properties our results indicate different mechanisms for oxidative stress from CW and mode-locked laser exposure.
引用
收藏
页码：150 / 155
页数：6
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