Confocal spectral imaging in tissue with contrast provided by Raman vibrational signatures

被引：11
|
作者：
Whitley, Andrew [1 ]
Adar, Fran [1 ]
机构：
[1] Horiba Jobin Yvon, Edison, NJ 08820 USA
来源：
CYTOMETRY PART A | 2006年 / 69A卷 / 08期
关键词：
Raman microscopy; confocal chemical mapping;
D O I：
10.1002/cyto.a.20298
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Confocal Raman imaging of biological materials offers the opportunity to extract chemical information on histologically defined regions and on sub-cellular organelles. This article reviews the technology and some successful applications. The chemical contrast from vibrational Raman spectroscopy is derived from the specific atomic motion of every molecule as detected by the Raman phenomenon. Examples show the successful identification of foreign material in pathological specimens, identification of lipid-type and calcium mineral-type in a mouse model of atherosclerosis, and component mapping in a pharmaceutical tablet. It is suggested that these methods can even be useful in studying metabolic disorders. (c) 2006 International Society for Analytical Cytology.
引用
收藏
页码：880 / 887
页数：8
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