Serotyping, stx2 subtyping, and characterization of the locus of enterocyte effacement island of shiga toxin-producing Escherichia coli and E-coli O157:H7 strains isolated from the environment in France

被引:29
|
作者
Vernozy-Rozand, C
Montet, MP
Bertin, Y
Trably, F
Girardeau, JP
Martin, C
Livrelli, V
Beutin, L
机构
[1] Ecole Natl Vet Lyon, Unite Microbiol Alimentaire & Previsionnelle, F-69280 Marcy Letoile, France
[2] INRA, Microbiol Lab, Ctr Rech, F-63122 St Genes Champanelle, France
[3] Univ Auvergne Clermont 1, Grp Rech Pathogenie Bacterienne Intestinale, Fac Pharm, F-63001 Clermont Ferrand, France
[4] Robert Koch Inst, Div Microbial Toxins, D-13353 Berlin, Germany
关键词
D O I
10.1128/AEM.70.4.2556-2559.2004
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Twenty-seven Shiga toxin-producing Escherichia coli (STEC) strains were isolated from 207 stx-positive French environmental samples. Ten of these strains were positive for stx(1), and 24 were positive for stx(2) (10 were positive for stx(2vh-a) or Stx(2vh-b), 19 were positive for stx(2d), and 15 were positive for stx(2e)). One strain belonged to serotype O157:H7, and the others belonged to serogroups O2, O8, O11, O26, O76, O103, O113, O121, O141, O166, and O174. The environment is a reservoir in which new clones of STEC that are pathogenic for humans can emerge.
引用
收藏
页码:2556 / 2559
页数:4
相关论文
共 50 条
  • [1] The locus of enterocyte effacement pathogenicity island of shiga toxin-producing Escherichia coli O157:H7 and other attaching and effacing E-coli
    Kaper, JB
    JAPANESE JOURNAL OF MEDICAL SCIENCE & BIOLOGY, 1998, 51 : S101 - S107
  • [2] Characterization of Shiga toxin-producing Escherichia coli O157:H7 isolated in Italy and in France
    Giammanco, GM
    Pignato, S
    Grimont, F
    Grimont, PAD
    Caprioli, A
    Morabito, S
    Giammanco, G
    JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (12) : 4619 - 4624
  • [3] Characterization of Escherichia coli O157:H7 and other Shiga toxin-producing E-coli serotypes isolated from sheep
    Kudva, IT
    Hatfield, PG
    Hovde, CJ
    JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (04) : 892 - 899
  • [4] Genetic characterization of Escherichia coli O157:H7/- strains carrying the stx2 gene but not producing Shiga toxin 2
    Koitabashi, T
    Vuddhakul, V
    Radu, S
    Morigaki, T
    Asai, N
    Nakaguchi, Y
    Nishibuchi, M
    MICROBIOLOGY AND IMMUNOLOGY, 2006, 50 (02) : 135 - 148
  • [5] Structure of Shiga toxin type 2 (Stx2) from Escherichia coli O157:H7
    Fraser, ME
    Fujinaga, M
    Cherney, MM
    Melton-Celsa, AR
    Twiddy, EM
    O'Brien, AD
    James, MNG
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (26) : 27511 - 27517
  • [6] Escherichia coli O157:H7 and other Shiga toxin-producing E-coli in white veal calves
    Cristancho, Laura
    Johnson, Roger P.
    McEwen, Scott A.
    Gyles, Carlton L.
    VETERINARY MICROBIOLOGY, 2008, 126 (1-3) : 200 - 209
  • [7] Shiga toxin-producing Escherichia coli O157:H7 in Japan
    Terajima, J
    Izumiya, H
    Wada, A
    Tamura, K
    Watanabe, H
    EMERGING INFECTIOUS DISEASES, 1999, 5 (02) : 301 - 302
  • [8] Relationship between stx Genotype and Stx2 Expression Level in Shiga Toxin-Producing Escherichia coli O157 Strains
    Kawano, Kimiko
    Ono, Hidetoshi
    Iwashita, Osamu
    Kurogi, Mai
    Haga, Takeshi
    Maeda, Ken
    Goto, Yoshitaka
    JAPANESE JOURNAL OF INFECTIOUS DISEASES, 2012, 65 (04) : 322 - 325
  • [9] Evolution of enterohemorrhagic Escherichia coli hemolysin plasmids and the locus for enterocyte effacement in Shiga toxin-producing E-coli
    Boerlin, P
    Chen, S
    Colbourne, JK
    Johnson, R
    De Grandis, S
    Gyles, C
    INFECTION AND IMMUNITY, 1998, 66 (06) : 2553 - 2561
  • [10] Use of ramification amplification assay for detection of Escherichia coli O157:H7 and other E-coli Shiga toxin-producing strains
    Li, F
    Zhao, CY
    Zhang, WD
    Cui, SH
    Meng, JH
    Wu, J
    Zhang, DY
    JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (12) : 6086 - 6090