Adaptation of infectious bursal disease virus in chicken embryo fibroblast cell culture and its fine structure

被引：0
|
作者：
Kumar, R [1 ]
Chandra, R
Rao, VDP
Shukla, SK
机构：
[1] Govind Ballabh Pant Krishi Evam Praudyogik Vishva, Dept Microbiol, Pantnagar 263145, Uttar Pradesh, India
[2] Govind Ballabh Pant Krishi Evam Praudyogik Vishva, Coll Vet Sci, Dept Med, Pantnagar 263145, Uttar Pradesh, India
来源：
INDIAN JOURNAL OF ANIMAL SCIENCES | 2000年 / 70卷 / 02期
关键词：
chicken embryo fibroblast cells; electron microscopy; infectious bursal disease; natural outbreaks;
D O I：
暂无
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
Two outbreaks of suspected infectious bursal disease (IBD) at university poultry farms were investigated. On the basis of epidemiological data, characteristic gross and microscopic changes in different organs including bursa of Fabricius (BF) and spleen, presence of IBD virus antigen in BF and demonstration of intracytoplasmic virus particles of 59.2 nm diameter in infected chicken embryo fibroblast cells by transmission electron microscopy confirmed the outbreak as IBD. The type and distribution of lesions and mortality up to 23.2% is highly suggestive of involvement of IBD virus of moderate virulence. Neutralization of cell culture grown virus with reference antisera revealed the identity of virus as IBDV serotype 1.
引用
收藏
页码：110 / 113
页数：4
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