Formation of actin networks in Microfluidic concentration gradients

被引:10
|
作者
Strelnikova, Natalja [1 ]
Herren, Florian [1 ]
Schoenenberger, Cora-Ann [1 ]
Pfohl, Thomas [1 ]
机构
[1] Univ Basel, Dept Chem, Basel, Switzerland
来源
FRONTIERS IN MATERIALS | 2016年 / 3卷
基金
瑞士国家科学基金会;
关键词
microfluidics; filaments; bundling; heterogeneous networks; FEM simulations; F-ACTIN; FILAMENTS; MICRORHEOLOGY; PLATFORMS; MIGRATION;
D O I
10.3389/fmats.2016.00020
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
The physical properties of cytoskeletal networks are contributors in a number of mechanical responses of cells, including cellular deformation and locomotion, and are crucial for the proper action of living cells. Local chemical gradients modulate cytoskeletal functionality including the interactions of the cytoskeleton with other cellular components. Actin is a major constituent of the cytoskeleton. Introducing a microfluidic-based platform, we explored the impact of concentration gradients on the formation and structural properties of actin networks. Microfluidic-controlled flow-free and steady-state experimental conditions allow for the generation of chemical gradients of different profiles, such as linear or step-like. We discovered specific features of actin networks emerging in defined gradients. In particular, we analyzed the effects of spatial conditions on network properties, bending rigidities of network links, and the network elasticity.
引用
收藏
页数:9
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