Isolation of mRNAs induced by a hazardous chemical in white-rot fungus, Coriolus versicolor, by differential display

White-rot fungus Coriolus versicolor, a ligninolytic basidiomycete, has been studied because of its ability to degrade hazardous chemicals, In this study, we searched for genes that are induced by a hazardous chemical using the mRNA differential-display technique and C. versicolor IFO30340 that has been exposed to pentachlorophenol (PCP), Five cDNA fragments were cloned and the DNA sequences of two fragments were analyzed in further detail, The clones corresponded to novel genes that have not previously been identified in C. versicolor. One of the cDNAs exhibited strong sequence homology to the gene for an enolase and the other exhibited homology to a heat shock protein, The expression of the two genes was up-regulated in PCP-treated C. versicolor. (C) 1997 Federation of European Biochemical Societies.