Selection of DNA Aptamers for Subcellular Localization of RBSDV P10 Protein in the Midgut of Small Brown Planthoppers by Emulsion PCR-Based SELEX

被引：5

作者：

Liu, Haoqiu ^{[1
,2
]}

Zhou, Yijun ^{[3
]}

Xu, Qiufang ^{[3
]}

Wong, Sek-Man ^{[1
,2
,4
]}

机构：

[1] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore

[2] Natl Univ Singapore, Suzhou Res Inst, Suzhou 215123, Peoples R China

[3] Jiangsu Acad Agr Sci, Inst Plant Protect, State Key Lab Breeding Base, Key Lab Food Qual & Safety Jiangsu Prov, Nanjing 210014, Peoples R China

[4] Temasek Life Sci Lab, 1 Res Link, Singapore 117604, Singapore

来源：

VIRUSES-BASEL | 2020年 / 12卷 / 11期

基金：

中国国家自然科学基金;

关键词：

aptamers; rice black-streaked dwarf virus; RBSDV; SELEX; emulsion PCR; BLACK-STREAKED-DWARF; POLYMERASE-CHAIN-REACTION; NUCLEIC-ACID APTAMERS; EXPONENTIAL ENRICHMENT; SYSTEMATIC EVOLUTION; SEQUENCE-ANALYSIS; RAPID DETECTION; RICE; VIRUS; MAIZE;

D O I：

10.3390/v12111239

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Rice black-streaked dwarf virus (RBSDV), classified under the Reoviridae, Fijivirus genus, caused an epidemic in the eastern provinces of China and other East Asian countries and resulted in severe yield loss in rice and wheat production. RBSDV is transmitted by the small brown planthopper (SBPH, Laodelphax striatellus Fallen) in a persistent manner. In order to provide a stable and cost-effective detection probe, in this study we selected three DNA aptamers (R3, R5 and R11) by an optimized, standardized and time saving emulsion PCR-based SELEX, for the detection of RBSDV outer-shell P10 protein for in situ localization studies in the midgut of SBPH. The specificity of these three DNA aptamers was tested through detection of the P10 protein using an enzyme-linked oligonucleotide assay (ELONA) and aptamer-based dot-blot ELISA. All three DNA aptamers can be used to detect RBSDV P10 protein by immunofluorescent labeling in the midgut of RBSDV-infected SBPH. These data show that the selected aptamers can be used for the detection of RBSDV P10 protein in vitro and in vivo. This is the first report of aptamers being selected for detection of a rice virus capsid protein.

引用

页数：19