A binary DNA probe for highly specific nucleic acid recognition

被引：106
|
作者：
Kolpashchikov, Dmitry M. [1 ]
机构：
[1] Columbia Univ, Dept Med, Div Expt Therapeut, New York, NY 10032 USA
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 32期
关键词：
MOLECULAR BEACONS; DISCRIMINATION; HYBRIDIZATION; AFFINITY;
D O I：
10.1021/ja0628093
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A new concept for nucleic acid probe design is reported. The extremely high selectivity of the probe is predetermined by cooperative hybridization of the two relatively short ( 10 nucleotide) DNA hairpin fragments to the analyte. A binary DNA probe fluorescently reports the presence of 0.5% of the analyte in excess amount of a single base substituted oligodeoxyribonucleotide and distinguishes single nucleotide substitutions at any position of a 20-mer oligonucleotide at room temperature.
引用
收藏
页码：10625 / 10628
页数：4
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