Development of TaqMan real-time PCR for detection of stenotrophomonas maltophilia

In order to establish efficient and rapid real-time PCR to detect Stenotrophomonas maltophilia, specific primers and probe were designed according to the 16SrRNA conservative gene sequence of S. maltophilia. Proper TaqMan real-time PCR technique of S. maltophilia was developed by optimizing reaction conditions, and performing the sensitivity, specificity, reproducibility test and clinical samples identification. Our results showed the standard had a good linear relationship at the concentration of 1.12x10(7) to 1.12x10(2) copies/mu L, and the minimum detectable concentration was 1.12x10(-2) copies/mu L, also the method has no direct cross transmission from 21 kinds of bacteria and viruses. The CV values of intra-groups and inter-group is all less than 3%. All tests and clinical samples test showed that the method had many advantages, such as strong specificity, high sensitivity, good stability, high veracity and fast detection. Hence, TaqMan real-time PCR can be used for early diagnosis, rapid detection and quantitative analysis of samples in S. maltophilia's infection.