Progress on Application of Conditional System in Gene-editing Pigs

Conditional gene-editing system can limit gene modification occur in specific tissues or organs at aspecific time,which can be realized by introducing conditional gene deletion system and conditional geneoverexpression system into animals,and has been widely used in the study of gene function in mice.Gene-editingpigs have important application value in agriculture and biology.In the field of biological medicine,because ofthe similarities of body,organ size,physiology,metabolism,and life with humans,genetically-modified pigs canbe used for the basic research of gene function,and as a model of human disease,xenotransplantation supply andhuman functional protein bioreactor.Benefit from the development of gene editing technology in recent years,itis possible to introduce the conditional gene expression system in pigs,and conditional gene expression pig modelwill greatly promote the research progress.In this paper,we review the progress in the construction of conditionalmodification systems and genetically-modified pig models by using gene editing techniques