Detecting Infectious Organisms: A Concerted Approach using Genomics, Molecular Engineering and Nano-enabled bio-MEMS Technologies

被引：0

作者：

Keegan, N. ^{[1
]}

Spoors, J. ^{[1
]}

Flanagan, K. ^{[1
]}

Cockell, S. ^{[1
]}

Gendra, E. ^{[2
]}

Ferrigno, P. Ko ^{[2
]}

Hedley, J. ^{[1
]}

Wipat, A. ^{[1
]}

Harwood, C. ^{[1
]}

McNeil, C. J. ^{[1
]}

机构：

[1] Newcastle Univ, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England

[2] Aptuscan Ltd, Leeds Innovat Ctr, Leeds LS2 9DF, W Yorkshire, England

来源：

NANOTECHNOLOGY 2011: BIO SENSORS, INSTRUMENTS, MEDICAL, ENVIRONMENT AND ENERGY, NSTI-NANOTECH 2011, VOL 3 | 2011年

关键词：

genomics; peptide aptamer (Scannin); bioMEMS; bacteria; clinical diagnostics; PROTEIN-DETECTION; PEPTIDE APTAMERS;

D O I：

暂无

中图分类号：

TE [石油、天然气工业]; TK [能源与动力工程];

学科分类号：

0807 ; 0820 ;

摘要：

Development of technology/workflows to detect target organisms within minutes, are being evaluated using Methicillin Resistant Staphylococcus aureus (MRSA) as a proof of principle test case. Bacterial genomics and powerful bioinformatics identify unique surface layer proteins, informing molecular engineers of a target sequence against which to produce peptide aptamers (lead time 12 weeks). The peptide aptamers have engineered tethers within their sequence for site specific immobilisation at sensor interfaces and subsequent recognition of their target protein. In parallel microelectromechanical systems (MEMS) sensors are being fabricated with the aim of capture and detection of target bacteria with little or no sample processing.

引用

页码：64 / 67

页数：4