Micropropagation of Anethum graveolens L through axillary shoot proliferation

被引：7
|
作者：
Sharma, RK [1 ]
Wakhlu, AK
Boleria, M
机构：
[1] SP Coll, Dept Bot, Srinagar 190001, Jammu & Kashmir, India
[2] Univ Jammu, Dept Bot, Plant Tissue Culture Lab, Jammu 180006, India
来源：
JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY | 2004年 / 13卷 / 02期
关键词：
axillary shoot proliferation; essential oil; herbal medicine;
D O I：
10.1007/BF03263214
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A protocol is described for rapid and large-scale in vitro propagation of Anethum graveolens by enhanced axillary shoot induction that was dependent on BAP supply. The synergistic combination of 0.5 mg l(-1) BAP and 0.1 mg l(-1) IBA induced 100% shoot formation as well as shoot number (6.6+/-0.48 per explant). Subculturing of shoot tips of in vitro plants on multiplication medium enabled continuous production of healthy shoots with similar frequency. Rooting of shoots was achieved on a medium with 1 mg l(-1) IBA and 0.5 mg l(-1) Kn. Micropropagated plants established in garden were uniform and identical to the donor plant with respect to morphological and cytological characteristics.
引用
收藏
页码：157 / 159
页数：3
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