Echogenic, Ultrasound-Sensitive Chitosan Nanodroplets for Spatiotemporally Controlled DKK-2 Gene Delivery to Prostate Cancer Cells

被引：11

作者：

Liu, Xinxin ^{[1
]}

Shi, Dandan ^{[1
]}

Guo, Lu ^{[1
]}

Zhou, Xiaoying ^{[1
]}

Shang, Mengmeng ^{[1
]}

Sun, Xiao ^{[1
]}

Meng, Dong ^{[1
]}

Zhao, Yading ^{[1
]}

Li, Jie ^{[1
]}

机构：

[1] Shandong Univ, Dept Ultrasound, Qilu Hosp, Wenhua Western Rd,Jinan 107, Jinan, Peoples R China

来源：

INTERNATIONAL JOURNAL OF NANOMEDICINE | 2021年 / 16卷

基金：

中国国家自然科学基金;

关键词：

nanodroplets; chitosan; DKK-2; ultrasonic gene transfer; prostate cancer; NANOPARTICLES; MICELLES; THERAPY; GROWTH;

D O I：

10.2147/IJN.S286474

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Purpose: To synthesize echogenic chitosan/perfluorohexane nanodroplets (CNDs) for DKK-2 gene delivering in a spatiotemporally controlled manner in vitro. Methods: The characteristics, contrast-enhanced ultrasound imaging, DNA binding and DNase protection capacity, DKK-2 gene transfection and effects on LNCaP cells of these CNDs were investigated. Results: The obtained CNDs showed positive surface charges and could attract the genetic cargo with negative surface charges to form nanocomplexes. Agarose gel electrophoresis confirmed binding of the CNDs and pDNA. DKK-2 pDNA-loaded CNDs, in combination with ultrasound, ruptured and released DKK-2 pDNA, entering LNCaP cells through nano-scale pores in the cell membrane, which further reduced the proliferation of LNCaP cells. Conclusion: These stable and safe CNDs may be a promising choice to achieve efficient ultrasound-mediated gene delivery to specific tissues in a spatiotemporally controlled manner.

引用

页码：421 / 432

页数：12

共 9 条

[1] Echogenic Chitosan Nanodroplets for Spatiotemporally Controlled Gene Delivery
Yu, Jinsui
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[2] Chitosan Based AGR2 siRNA Nanoparticle Delivery System for Prostate Cancer Cells
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不详
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