Decellularized Avian Cartilage, a Promising Alternative for Human Cartilage Tissue Regeneration

被引:5
|
作者
Ayariga, Joseph Atia [1 ]
Huang, Hanxiao [1 ]
Dean, Derrick [1 ]
机构
[1] Alabama State Univ, Coll Sci Technol Engn & Math C STEM, Biomed Engn Program, 1627 Hall St, Montgomery, AL 36104 USA
基金
美国国家科学基金会;
关键词
chondrocytes; extracellular matrix; cartilage; decellularization; scaffold; EXTRACELLULAR-MATRIX SCAFFOLDS; ALKALINE-PHOSPHATASE; PORCINE KIDNEYS; HOST RESPONSE; CELL THERAPY; ALAMAR BLUE; FOLLOW-UP; IN-VIVO; BONE; PROLIFERATION;
D O I
10.3390/ma15051974
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Articular cartilage defects, and subsequent degeneration, are prevalent and account for the poor quality of life of most elderly persons; they are also one of the main predisposing factors to osteoarthritis. Articular cartilage is an avascular tissue and, thus, has limited capacity for healing and self-repair. Damage to the articular cartilage by trauma or pathological causes is irreversible. Many approaches to repair cartilage have been attempted with some potential; however, there is no consensus on any ideal therapy. Tissue engineering holds promise as an approach to regenerate damaged cartilage. Since cell adhesion is a critical step in tissue engineering, providing a 3D microenvironment that recapitulates the cartilage tissue is vital to inducing cartilage regeneration. Decellularized materials have emerged as promising scaffolds for tissue engineering, since this procedure produces scaffolds from native tissues that possess structural and chemical natures that are mimetic of the extracellular matrix (ECM) of the native tissue. In this work, we present, for the first time, a study of decellularized scaffolds, produced from avian articular cartilage (extracted from Gallus Gallus domesticus), reseeded with human chondrocytes, and we demonstrate for the first time that human chondrocytes survived, proliferated and interacted with the scaffolds. Morphological studies of the decellularized scaffolds revealed an interconnected, porous architecture, ideal for cell growth. Mechanical characterization showed that the decellularized scaffolds registered stiffness comparable to the native cartilage tissues. Cell growth inhibition and immunocytochemical analyses showed that the decellularized scaffolds are suitable for cartilage regeneration.
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页数:34
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