A Comparison of Two Cryogenic Protocols in Cryopreservation of Apple Shoot Tips

被引:5
|
作者
Feng, C. H. [1 ]
Li, B. Q. [1 ]
Hu, L. Y. [1 ]
Wang, M. R. [1 ]
Wang, Q. C. [1 ]
机构
[1] Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China
关键词
apple; encapsulation-dehydration; droplet-vitrification; shoot tips; recovery; histological studies; ENCAPSULATION-DEHYDRATION; BUDS; PEAR;
D O I
10.17660/ActaHortic.2014.1039.20
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Apple (Malus) is one of the most important fruit crops globally. Recently, two more efficient cryopreservation protocols: encapsulation-dehydration (Feng et al., 2013) and droplet-vitrification (Halmagyi et al., 2010), have been reported for apple shoot tips and appeared to be promising protocols applicable for long-term conservation of a wide range of apple genetic resources. The present study attempted to compare these two protocols in terms of survival and shoot regrowth rates, time required for recovery and number of survival cells in shoot tips following cryopreservation. Results obtained from the present study would provide valuable information on setting-up cryobanking of apple genetic resources and cryotherapy for apple virus eradication.
引用
收藏
页码:161 / 166
页数:6
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