Electrically induced brain-derived neurotrophic factor release from schwann cells

被引:37
|
作者
Luo, Beier [1 ]
Huang, Jinghui [2 ]
Lu, Lei [3 ]
Hu, Xueyu [2 ]
Luo, Zhuojing [2 ]
Li, Ming [1 ]
机构
[1] Second Mil Med Univ, Inst Orthopaed, Changhai Hosp, Shanghai, Peoples R China
[2] Fourth Mil Med Univ, Inst Orthopaed, Xijing Hosp, Xian 710032, Peoples R China
[3] Fourth Mil Med Univ, Dept Oral Anat & Physiol, Sch Stomatol, Xian 710032, Peoples R China
基金
中国国家自然科学基金;
关键词
calcium; brain-derived neurotrophic factor; mitogen-activated protein kinase; electrical stimulation; calcium-calmodulin dependent protein kinase; CONDUCTIVE POLYPYRROLE/CHITOSAN POLYMERS; OLFACTORY ENSHEATHING CELLS; PERIPHERAL-NERVE; SPINAL-CORD; HIPPOCAMPAL-NEURONS; DEPENDENT RELEASE; REGENERATION; BDNF; FIELDS; NGF;
D O I
10.1002/jnr.23365
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Regulating the production of brain-derived neurotrophic factor (BDNF) in Schwann cells (SCs) is critical for their application in traumatic nerve injury, neurodegenerative disorders, and demyelination disease in both central and peripheral nervous systems. The present study investigated the possibility of using electrical stimulation (ES) to activate SCs to release BDNF. We found that short-term ES was capable of promoting BDNF production from SCs, and the maximal BDNF release was achieved by ES at 6 V (3 Hz, 30 min). We further examined the involvement of intracellular calcium ions ([Ca2+](i)) in the ES-induced BDNF production in SCs by pharmacological studies. We found that the ES-induced BDNF release required calcium influx through T-type voltage-gated calcium channel (VGCC) and calcium mobilization from internal calcium stores, including inositol triphosphate-sensitive stores and caffeine/ryanodine-sensitive stores. In addition, calcium-calmodulin dependent protein kinase IV (CaMK IV), mitogen-activated protein kinase (MAPK), and cAMP response element-binding protein (CREB) were found to play important roles in the ES-induced BDNF release from SCs. In conclusion, ES is capable of activating SCs to secrete BDNF, which requires the involvement of calcium influx through T-type VGCC and calcium mobilization from internal calcium stores. In addition, activation of CaMK IV, MAPK, and CREB were also involved in the ES-induced BDNF release. The findings indicate that ES can improve the neurotrophic ability in SCs and raise the possibility of developing electrically stimulated SCs as a source of cell therapy for nerve injury in both peripheral and central nervous systems. (c) 2014 Wiley Periodicals, Inc.
引用
收藏
页码:893 / 903
页数:11
相关论文
共 50 条
  • [1] Brain-derived neurotrophic factor suppresses anoikis-induced death of Schwann cells
    Koda, Masao
    Someya, Yukio
    Nishio, Yutaka
    Kadota, Ryo
    Mannoji, Chikato
    Miyashita, Tomohiro
    Okawa, Akihiko
    Murata, Atsushi
    Yamazaki, Masashi
    NEUROSCIENCE LETTERS, 2008, 444 (02) : 143 - 147
  • [2] Brain-derived neurotrophic factor suppresses anoikis-induced cell death of schwann cells
    Koda, M
    Nishio, Y
    Someya, Y
    Kadota, R
    Mannoji, C
    Miyashita, T
    Okawa, A
    Yoshinaga, K
    Moriya, H
    Yamazaki, M
    JOURNAL OF NEUROTRAUMA, 2005, 22 (10) : 1236 - 1236
  • [3] Brain-derived neurotrophic factor suppresses anoikis-induced cell death of Schwann
    Koda, M
    Someya, Y
    Nishio, Y
    Kadota, R
    Mannoji, C
    Miyashita, T
    Okawa, A
    Moriya, H
    Yamazaki, M
    JOURNAL OF NEUROTRAUMA, 2006, 23 (05) : 798 - 798
  • [4] Evidence for a release of brain-derived neurotrophic factor from the brain during exercise
    Rasmussen, Peter
    Brassard, Patrice
    Adser, Helle
    Pedersen, Martin V.
    Leick, Lotte
    Hart, Emma
    Secher, Niels H.
    Pedersen, Bente K.
    Pilegaard, Henriette
    EXPERIMENTAL PHYSIOLOGY, 2009, 94 (10) : 1062 - 1069
  • [5] Brain-derived neurotrophic factor
    Binder, DK
    Scharfman, HE
    GROWTH FACTORS, 2004, 22 (03) : 123 - 131
  • [6] Brain-derived neurotrophic factor
    不详
    NATURE REVIEWS DRUG DISCOVERY, 2011, 10 (03) : 169 - 169
  • [7] BRAIN-DERIVED NEUROTROPHIC FACTOR
    BARDE, YA
    THOENEN, H
    JOURNAL OF CELLULAR BIOCHEMISTRY, 1987, : 171 - 171
  • [8] Secretion of brain-derived neurotrophic factor from brain microvascular endothelial cells
    Wang, H
    Ward, N
    Boswell, M
    Katz, DM
    EUROPEAN JOURNAL OF NEUROSCIENCE, 2006, 23 (06) : 1665 - 1670
  • [9] Exogenous gangliosides increase the release of brain-derived neurotrophic factor
    Lim, Seung T.
    Esfahani, Kamilla
    Avdoshina, Valeriya
    Mocchetti, Italo
    NEUROPHARMACOLOGY, 2011, 60 (7-8) : 1160 - 1167
  • [10] Enhancement of neurotransmitter release induced by brain-derived neurotrophic factor in cultured hippocampal neurons
    Li, YX
    Zhang, YO
    Lester, HA
    Schuman, EM
    Davidson, N
    JOURNAL OF NEUROSCIENCE, 1998, 18 (24): : 10231 - 10240