Experimental Studies of transplantation of human amnion membrane mesenchymal stem cells for cerebral ischemia -reperfusion injury in rats

Objective An established mouse ischemia-reperfusion model will be used to assess the actions and related mechanisms of cerebral ischemia injuries via different pathways of transplanting hAMSCs. Methods Using modified suture method, the rat models of middle cerebral artery occlusion reperfusion were established. hAMSCs were transplanted by ventriculus lateralis cerebri and tail vein after 24 hours cerebral ischemia. Neurological Severity Score (NSS) was evaluated after 1 week and 4weeks cerebral ischemia. Immunofluorescent studies were carried out for detecting expression of BrdU positive cells.The volume of cerebral infarction was detected after 1 week cerebral ischemia. The results were evaluated on different transplanted pathways. Results hAMSCs transplanted via different pathways have been promoted on local survival and differentiation so that greatly improved animal nerve functions. The results showed transplanting pathway from ventriculus lateralis cerebri has better effects than from tail vein. Conclusion hAMSCs could effectively promote animal nerve functions.