Pseudonocardia parietis sp nov., from the indoor environment

A Gram-positive, rod-shaped, non-endospore-forming, mycelium-forming actinobacterium (04-St-002(T)) was isolated from the wall of an indoor environment colonized with moulds. On the basis of 16S rRNA gene sequence similarity studies, strain 04-St-002(T) was shown to belong to the family Pseudonocardiaceae, and to be most closely related to Pseudonocardia antarctica (99.2 %) and Pseudonocardia alni (99.1 %). The major menaquinones were MK-8(H-4) and MK-8(H-2), the phospholipid type was PIII, and the polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylmonomethylethanolamine and phosphatidylcholine, Moderate amounts of phosphatidylglycerol and an unknown polar lipid, L1, small or trace amounts of phosphatidylinositol, phosphatidylinositol-mannoside, three unknown lipids, two unknown phospholipids and four unknown glycolipids were also detected. The major fatty acids iso-C-16:0, iso-C-16:1, C-16:0 and C-16:1 omega 7c supported the affiliation of strain 04-St-002(T) to the genus Pseudonocardia. The results of DNA-DNA hybridization, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 04-St-002(T) from the two most closely related species, P. alni and P. antarctica. Strain 04-St-002(T) represents a novel species, for which the name Pseudonocardia parietis sp. nov. is proposed. The type strain is 04-St-002(T) (=DSM 45256(T)= CCM 7582(T)).