Dark-field illumination in conjunction with confocal Raman spectroscopy for real-time noninvasive aqueous humor investigation

被引：1

作者：

Zhang, Shuo ^{[1
]}

Erckens, Roel J. ^{[1
]}

Jongsma, Frans H. M. ^{[1
]}

De Brabander, John ^{[1
]}

Webers, Carroll A. B. ^{[1
]}

Berendschot, Tos T. J. M. ^{[1
]}

机构：

[1] Univ Eye Clin Maastricht, Maastricht, Netherlands

来源：

OPTICAL ENGINEERING | 2020年 / 59卷 / 09期

关键词：

Raman spectroscopy; eye; aqueous humor; confocal microscopy; dark-field illumination; TISSUES; GLUCOSE;

D O I：

10.1117/1.OE.59.9.092002

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

To obtain a real-time noninvasive analysis of the content of the aqueous humor (AH) in the eye, a confocal dark-field ophthalmic probe was designed and optimized by ray-tracing software. The confocal technique allows Raman spectrometric sampling of the AH with suppressing signals from the surrounding tissues. Dark-field illumination prevents the excitation light to reach the vulnerable retinal tissue directly. In order to evaluate the optical performance and safety of this method, a functional prototyped eye contact probe has been designed and tested on ex vivo rabbit eyes. (C) 2020 Society of Photo-Optical Instrumentation Engineers (SPIE)

引用

页数：9

共 29 条

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