Dynamics of the accumulation of thiamine during maturation of soybean seeds

The dynamics of the accumulation of thiamine were followed in developing seeds of four genotypes of soybean under defined climatic and constant topographical and agrotechnical conditions in the course of three consecutive vegetation years. Tests were carried out at weekly intervals until the seeds were completely mature and had a moisture content of about 14 %. Thiamine content was determined fluorometrically by measuring the intensity of thiocrome fluorescence. It was found that the greatest accumulation of the dry matter took place during the seed development stages III and IV whereas thiamine accumulation is more or less linear during the development. Maximal concentrations of thiamine were found in mature seeds. Although the dynamics of accumulation, regardless of the year of investigation, were the same in all the genotypes, the analysis of the impact of climatic factors showed that higher precipitation during the period of seed development, along with moderate air temperature, affected thiamine accumulation of some soybean genotypes more than others.