Outer membrane vesicle vaccine from Vibrio cholerae is still a promise: Proteomic characterization

被引：0

作者：

Martinez-Cabrera, Ileana ^{[1
]}

de los Angeles Padron-Collazo, Maria ^{[1
]}

Wheeler, Jun X. ^{[2
]}

Luis Perez-Quinoy, Jose ^{[1
]}

Garcia Imia, Luis ^{[1
]}

Gonzalez, Yamisley ^{[1
]}

Acosta, Michel ^{[1
]}

Adonis Cabrera-Arias, Ruben ^{[1
]}

Ano, Gemma ^{[1
]}

Cedre, Barbara ^{[1
]}

Valmaseda, Tania ^{[1
]}

Aranguren, Yisabel ^{[1
]}

Alvarez, Maydelis ^{[1
]}

Serrano-Hernandez, Daily ^{[1
]}

Milian, Ernesto ^{[1
]}

Farinas, Mildrey ^{[1
]}

Talavera-Coronel, Arturo ^{[1
]}

Garcia Sanchez, Hilda ^{[1
]}

Riveron-Martinez, Luis ^{[1
]}

Sierra Gonzalez, Victoriano Gustavo ^{[3
]}

Jones, Christopher ^{[2
]}

机构：

[1] Inst Finlay Vacunas, Ave 27, Havana 19805, Cuba

[2] Natl Inst Biol Stand & Control Reino Unido, Ridge, Herts, England

[3] BioCubaFarma, Grp Ind Biotecnol & Farmaceut Cuba, Havana, Cuba

来源：

ACTA BIOQUIMICA CLINICA LATINOAMERICANA | 2019年 / 53卷 / 01期

关键词：

Vibrio cholerae; proteomics; vaccines; outer membrane proteins; PROTEIN; FLAGELLINS;

D O I：

暂无

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Cholera epidemics affect a large number of African, Asian and Caribbean countries. The climate changes and the constant migrations cause this disease to spread, making it is necessary to obtain protective vaccines. In the present work, a new vaccine of outer membrane vesicles (OMV) from V. cholerae O1 El Tor biotype Ogawa serotype strain C7258 at Finlay Institute of vaccines (Cuba) was characterized by proteomic methods. A total of 53 proteins present in the OMV (approximate ratio of 4 proteins by electrophoresis band) were identified, separated by one dimension electrophoresis and digested by tripsin method. The fragments were separated by high performance liquid chromatography (HPLC) coupled to mass spectrometry, sequenced and identified, using Swiss-Prot and TrEMBL protein databases. The pattern showed some proteins (12 cytoplasmic proteins and 5 outer membrane proteins) suggested within the highest quality proteome for vaccine candidate. The best conditions for proteins separation by two dimension electrophoresis were studied. The OMV composition was based on proteins described to the immunity response and protection against V cholerae O1 El Tor biotype Ogawa serotype.

引用

页码：43 / 51

页数：9

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