Patronin governs minus-end-out orientation of dendritic microtubules to promote dendrite pruning in Drosophila

被引：31

作者：

Wang, Yan ^{[1
,2
]}

Rui, Menglong ^{[1
]}

Tang, Quan ^{[1
,3
]}

Bu, Shufeng ^{[1
,3
]}

Yu, Fengwei ^{[1
,2
,3
,4
]}

机构：

[1] Natl Univ Singapore, Temasek Life Sci Lab, Singapore, Singapore

[2] Natl Univ Singapore, Ctr Life Sci, NUS Grad Sch Integrat Sci & Engn, Singapore, Singapore

[3] Natl Univ Singapore, Dept Biol Sci, Singapore, Singapore

[4] Natl Univ Singapore, Duke NUS Grad Med Sch, Neurosci & Behav Disorder Program, Singapore, Singapore

来源：

ELIFE | 2019年 / 8卷

基金：

新加坡国家研究基金会;

关键词：

BINDING-PROTEIN; UBIQUITIN-PROTEASOME; SENSORY NEURON; AXON; POLARITY; DYNAMICS; KATANIN; GROWTH; DEGENERATION; REGENERATION;

D O I：

10.7554/eLife.39964

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Class IV ddaC neurons specifically prune larval dendrites without affecting axons during Drosophila metamorphosis. ddaCs distribute the minus ends of microtubules (MTs) to dendrites but the plus ends to axons. However, a requirement of MT minus-end-binding proteins in dendrite-specific pruning remains completely unknown. Here, we identified Patronin, a minus-end-binding protein, for its crucial and dose-sensitive role in ddaC dendrite pruning. The CKK domain is important for Patronin's function in dendrite pruning. Moreover, we show that both patronin knockdown and overexpression resulted in a drastic decrease of MT minus ends and a concomitant increase of plus-end-out MTs in ddaC dendrites, suggesting that Patronin stabilizes dendritic minus-end-out MTs. Consistently, attenuation of Klp10A MT depolymerase in patronin mutant neurons significantly restored minus-end-out MTs in dendrites and thereby rescued dendrite-pruning defects. Thus, our study demonstrates that Patronin orients minus-end-out MT arrays in dendrites to promote dendrite-specific pruning mainly through antagonizing Klp10A activity.

引用

页数：29