Highly efficient in vitro adventitious shoot regeneration of peppermint (Mentha x piperita L.) using internodal explants

被引：5

作者：

Wang, Xiaohuan ^{[2
]}

Gao, Zhenhua ^{[2
]}

Wang, Yunzhen ^{[2
]}

Bressan, Ray A. ^{[3
]}

Weller, Stephen C. ^{[3
]}

Li, Xia ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Ctr Agr Resources, Inst Genet & Dev Biol, Shijiazhuang 050021, Hebei, Peoples R China

[2] Chinese Acad Sci, State Key Lab Plant Cell & Chromosome Engn, Inst Genet & Dev Biol, Ctr Agr Resources, Shijiazhuang 050021, Hebei, Peoples R China

[3] Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA

来源：

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT | 2009年 / 45卷 / 04期

关键词：

Peppermint; Mentha x piperita; Internodes; Organogenesis; In vitro regeneration; SPEARMINT; TRANSFORMATION; MINT;

D O I：

10.1007/s11627-008-9170-x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

An in vitro regeneration system with a 100% efficiency rate was developed in peppermint [Mentha x piperita] using 5- to 7-mm-long second internode stem segments of 3-wk-old stock plants. Shoots developed at sites of excision on stem fragments either directly from the cells or via primary calluses. The optimal medium for maximum shoot initiation and regeneration contained Murashige and Skoog (MS) salts, B5 vitamins, thidiazuron (TDZ, 11.35 mu M), ZT (4.54 mu M), 10% coconut water (CW), 20 g l(-1) sucrose, 0.75% agar, adjusted to pH 5.8. A frequency of 100% shoot initiation was achieved, with an average of 39 shoots per explant. This regeneration system is highly reproducible. The regenerated plants developed normally and were phenotypically similar to Black Mitcham parents.

引用

页码：435 / 440

页数：6

共 50 条

[1] Highly efficient in vitro adventitious shoot regeneration of peppermint (Mentha x piperita L.) using internodal explants
Xiaohuan Wang
Zhenhua Gao
Yunzhen Wang
Ray A. Bressan
Stephen C. Weller
Xia Li
[J]. In Vitro Cellular & Developmental Biology - Plant, 2009, 45
[2] In vitro adventitious shoot regeneration of native spearmint using internodal explants
Poovaiah, CR
Weller, SC
Jenks, MA
[J]. HORTSCIENCE, 2006, 41 (02) : 414 - 417
[3] Herbicide tests in peppermint (Mentha x piperita L.) in Thuringia
Schmatz, Rudiger
Schaekel, Christine
Dick, Christian
[J]. GESUNDE PFLANZEN, 2009, 61 (01): : 1 - 10
[4] Effect of clinorotation on in vitro cultured explants of Mentha piperita L.
Paolicchi, F
Mensuali-Sodi, A
Tognoni, F
[J]. SCIENTIA HORTICULTURAE, 2002, 92 (3-4) : 305 - 315
[5] Physiological postharvest response of peppermint (Mentha x piperita L.) herbs
Böttcher, H
Günther, I
Franke, R
[J]. GARTENBAUWISSENSCHAFT, 2002, 67 (06): : 243 - 254
[6] An Efficient Protocol for High-frequency Direct Multiple Shoot Regeneration from Internodes of Peppermint (Mentha x piperita)
Thul, Sanjog T.
Kukreja, Arun K.
[J]. NATURAL PRODUCT COMMUNICATIONS, 2010, 5 (12) : 1945 - 1946
[7] Herbicide tests in peppermint (Mentha x piperita L.) in Thuringia [Versuche mit Herbiziden in Pfefferminze Mentha x piperita L. in Thüringen]
Schmatz R.
Schäkel C.
Dick C.
[J]. Gesunde Pflanzen, 2009, 61 (1): : 1 - 10
[8] MORPHOLOGICAL AND GENETIC DIVERSITY AMONG PEPPERMINT (Mentha x piperita L.) CULTIVARS
Kieltyka-Dadasiewicz, Anna
Okon, Sylwia
Ociepa, Tomasz
Krol, Beata
[J]. ACTA SCIENTIARUM POLONORUM-HORTORUM CULTUS, 2017, 16 (03): : 151 - 161
[9] Chemical constituents, in vitro antibacterial and antifungal activity of Mentha x Piperita L. (peppermint) essential oils
Reddy, Desam Nagarjuna
Al-Rajab, Abdul Jabbar
Sharma, Mukul
Moses, Mylabathula Mary
Reddy, Gowkanapalli Ramachandra
Albratty, Mohammed
[J]. JOURNAL OF KING SAUD UNIVERSITY SCIENCE, 2019, 31 (04) : 528 - 533
[10] Phenolic Profile and Antioxidant Evaluation of Mentha x piperita L., (Peppermint) Extracts
Dorman, H. J. Damien
Kosar, Mueberra
Baser, K. Huesnue C.
Hiltunen, Raimo
[J]. NATURAL PRODUCT COMMUNICATIONS, 2009, 4 (04) : 535 - 542

← 1 2 3 4 5 →